- Datasheet
- Specific References
- Protocols
Overview
-
Product nameChicken IgY ELISA Kit
See all IgY kits -
Detection methodColorimetric
-
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample typeSerum, Plasma
-
Assay typeSandwich (quantitative)
-
Sensitivity1.531 ng/ml
-
Range6.25 ng/ml - 400 ng/ml
-
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay durationMultiple steps standard assay
-
Species reactivityReacts with: Chicken
-
Product overview
Abcam’s IgY Chicken ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of IgY in chicken serum or plasma samples.
In this assay the IgY present in samples reacts with the anti-IgY antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti- IgY antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgY. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgY in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgY in the test sample. The quantity of IgY in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Get results in 90 minutes with Chicken IgY ELISA Kit (ab189577) from our SimpleStep ELISA® range.
-
Tested applicationsSuitable for: Sandwich ELISAmore details
-
PlatformMicroplate
Properties
-
Storage instructionsPlease refer to protocols.
-
Components 1 x 96 tests 100X HRP-conjugated anti-chicken IgY antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chicken IgY Calibrator (Lyophilized) 1 vial Chicken IgY ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml Stop Solution 1 x 12ml -
Research areas
-
RelevanceSecreted immunoglobulins have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen. IgY has a tetrameric structure consisting of two identical light chains (Mr = 24 kDa) and two identical heavy chains (Mr = 73 kDa).
-
Alternative names
- FCR
- IGSF1
- immunoglobulin superfamily, member 1
-
Database links
- Entrez Gene: 419114 Chicken
Associated products
-
SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab157693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
-
Standard CurveRepresentative standard curve using ab157693 IgY Chicken ELISA Kit.
Protocols
References
ab157693 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"