Overview

  • Product name
    Chicken IgY ELISA Kit
    See all IgY kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1.531 ng/ml
  • Range
    6.25 ng/ml - 400 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Chicken
  • Product overview

    Abcam’s IgY Chicken ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of IgY in chicken serum or plasma samples.


     In this assay the IgY present in samples reacts with the anti-IgY antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti- IgY antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgY. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgY in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgY in the test sample. The quantity of IgY in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


    Get results in 90 minutes with Chicken IgY ELISA Kit (ab189577) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-chicken IgY antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chicken IgY Calibrator (Lyophilized) 1 vial
    Chicken IgY ELISA Microplate 1 unit
    Chromogen Substrate Solution 1 x 12ml
    Stop Solution 1 x 12ml
  • Research areas
  • Relevance
    Secreted immunoglobulins have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen. IgY has a tetrameric structure consisting of two identical light chains (Mr = 24 kDa) and two identical heavy chains (Mr = 73 kDa).
  • Alternative names
    • FCR
    • IGSF1
    • immunoglobulin superfamily, member 1
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab157693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab157693 IgY Chicken ELISA Kit.

Protocols

References

ab157693 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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