Overview

  • Product name
    Chicken Ovotransferrin ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Serum, Plasma, Other biological fluids
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.751 ng/ml
  • Range
    6.25 ng/ml - 400 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Chicken
  • Product overview

    Abcam’s Ovotransferrin Chicken kit is highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ovotransferrin in biological samples of chicken.

    In this assay the Ovotransferrin present in samples reacts with the anti-Ovotransferrin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Ovotransferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ovotransferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ovotransferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ovotransferrin in the test sample. The quantity of Ovotransferrin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-chicken Ovotransferrin antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chicken Ovotransferrin Calibrator (Lyophilized) 1 vial
    Chicken Ovotransferrin ELISA Microplate 1 unit
    Chromogen Substrate Solution 1 x 12ml
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. There are two forms of hen transferrin, ovotransferrin, found in the ovoducts and, serum transferrin, secreted by the liver. Serum transferrin may also have a role in stimulating cell proliferation and is regulated by iron levels. Ovotransferrin has a bacteriostatic function and, is not controlled by iron levels.
  • Sequence similarities
    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Post-translational
    modifications
    Different forms of hen transferrin are distinguished by their carbohydrate composition. Ovotransferrin and embryo serum transferrin but not adult serum transferrin, have bisecting N-acetylglucosamine. Transferrin secreted by embryo hepatocytes in primary culture is marked by the presence of (alpha1-6) fucosylation of the core N-acetylglucosamine. Serum transferrins also differ in the number of attached neuraminic acid residues. In both embryo forms, sialylation occurs on the Man (alpha 1-3)-linked antennae.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Allergen Gal d III
    • Conalbumin
    • lactotransferrin
    • LTF
    • Ovotransferrin
    • Serum transferrin
    • TF
    • TFEW
    • TRFE_CHICK
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab157694 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Standard Curve
      Standard Curve
      Representative standard curve using ab157694 Ovotransferrin Chicken ELISA Kit

    Protocols

    References

    ab157694 has not yet been referenced specifically in any publications.

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