Chicken Ovotransferrin ELISA Kit (ab157694)
- Datasheet
- References
- Protocols
Overview
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Product nameChicken Ovotransferrin ELISA Kit
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Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample typeSerum, Plasma, Other biological fluids
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Assay typeSandwich (quantitative)
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Sensitivity0.751 ng/ml
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Range6.25 ng/ml - 400 ng/ml
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Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Chicken
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Product overview
Abcam’s Ovotransferrin Chicken kit is highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ovotransferrin in biological samples of chicken.
In this assay the Ovotransferrin present in samples reacts with the anti-Ovotransferrin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Ovotransferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ovotransferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ovotransferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ovotransferrin in the test sample. The quantity of Ovotransferrin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 100X HRP-conjugated anti-chicken Ovotransferrin antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chicken Ovotransferrin Calibrator (Lyophilized) 1 vial Chicken Ovotransferrin ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml Stop Solution 1 x 12ml -
Research areas
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FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. There are two forms of hen transferrin, ovotransferrin, found in the ovoducts and, serum transferrin, secreted by the liver. Serum transferrin may also have a role in stimulating cell proliferation and is regulated by iron levels. Ovotransferrin has a bacteriostatic function and, is not controlled by iron levels.
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Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains. -
Post-translational
modificationsDifferent forms of hen transferrin are distinguished by their carbohydrate composition. Ovotransferrin and embryo serum transferrin but not adult serum transferrin, have bisecting N-acetylglucosamine. Transferrin secreted by embryo hepatocytes in primary culture is marked by the presence of (alpha1-6) fucosylation of the core N-acetylglucosamine. Serum transferrins also differ in the number of attached neuraminic acid residues. In both embryo forms, sialylation occurs on the Man (alpha 1-3)-linked antennae. -
Cellular localizationSecreted.
- Information by UniProt
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Alternative names
- Allergen Gal d III
- Conalbumin
- lactotransferrin
see all -
Database links
- Entrez Gene: 396241 Chicken
- SwissProt: P02789 Chicken
- Unigene: 2551 Chicken
Applications
Our Abpromise guarantee covers the use of ab157694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Standard CurveRepresentative standard curve using ab157694 Ovotransferrin Chicken ELISA Kit
Datasheets and documents
Protocols
References
ab157694 has not yet been referenced specifically in any publications.
