Chicken Ovotransferrin ELISA Kit (ab157694)

Submit a review Submit a question
Standard Curve

    Key features and details

    • Sensitivity: 0.751 ng/ml
    • Range: 6.25 ng/ml - 400 ng/ml
    • Sample type: Other biological fluids, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Chicken

    Overview

    • Product name

      Chicken Ovotransferrin ELISA Kit

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 10%

    • Sample type

      Serum, Plasma, Other biological fluids

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.751 ng/ml

    • Range

      6.25 ng/ml - 400 ng/ml

    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum > 85 % - %

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Chicken

    • Product overview

      Abcam’s Ovotransferrin Chicken kit is highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ovotransferrin in biological samples of chicken.

      In this assay the Ovotransferrin present in samples reacts with the anti-Ovotransferrin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Ovotransferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ovotransferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ovotransferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ovotransferrin in the test sample. The quantity of Ovotransferrin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X HRP-conjugated anti-chicken Ovotransferrin antibody 1 x 150µl
      20X Wash Buffer Concentrate 1 x 50ml
      5X Diluent Concentrate 1 x 50ml
      Chicken Ovotransferrin Calibrator (Lyophilized) 1 vial
      Chicken Ovotransferrin ELISA Microplate 1 unit
      Chromogen Substrate Solution 1 x 12ml
      Stop Solution 1 x 12ml

    • Research areas

    • Function

      Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. There are two forms of hen transferrin, ovotransferrin, found in the ovoducts and, serum transferrin, secreted by the liver. Serum transferrin may also have a role in stimulating cell proliferation and is regulated by iron levels. Ovotransferrin has a bacteriostatic function and, is not controlled by iron levels.

    • Sequence similarities

      Belongs to the transferrin family.
      Contains 2 transferrin-like domains.

    • Post-translational
      modifications

      Different forms of hen transferrin are distinguished by their carbohydrate composition. Ovotransferrin and embryo serum transferrin but not adult serum transferrin, have bisecting N-acetylglucosamine. Transferrin secreted by embryo hepatocytes in primary culture is marked by the presence of (alpha1-6) fucosylation of the core N-acetylglucosamine. Serum transferrins also differ in the number of attached neuraminic acid residues. In both embryo forms, sialylation occurs on the Man (alpha 1-3)-linked antennae.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Alternative names

      • Allergen Gal d III
      • Conalbumin
      • lactotransferrin
      • LTF
      • Ovotransferrin
      • Serum transferrin
      • TF
      • TFEW
      • TRFE_CHICK
      see all

    • Database links

      Associated products

        Images

        • Standard Curve
          Standard Curve
          Representative standard curve using ab157694 Ovotransferrin Chicken ELISA Kit

        References (0)

        Publishing research using ab157694? Please let us know so that we can cite the reference in this datasheet.

        ab157694 has not yet been referenced specifically in any publications.

        Customer reviews and Q&As

        Show All Reviews Q&A
        Submit a review Submit a question

        There are currently no Customer reviews or Questions for ab157694.
        Please use the links above to contact us or submit feedback about this product.

        Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
        For licensing inquiries, please contact partnerships@abcam.com