Product nameChicken Anti-Rabbit IgG H&L (Biotin)
See all IgG secondary antibodies
Tested applicationsSuitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
Rabbit IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Our Abpromise guarantee covers the use of ab6828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||1/1000 - 1/5000.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
This product has been referenced in:
- Sugawa F et al. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile. EMBO J 34:1009-24 (2015). IHC-P ; Human . Read more (PubMed: 25750208) »
- Takahashi N et al. Epithelial TRPV1 Signaling Accelerates Gingival Epithelial Cell Proliferation. J Dent Res 93:1141-7 (2014). Read more (PubMed: 25266715) »