Chicken Anti-Rabbit IgG H&L (Biotin) (ab6828)
Key features and details
- Chicken Anti-Rabbit IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Chicken
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr
Related conjugates and formulations
Overview
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Product name
Chicken Anti-Rabbit IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Chicken -
Target species
Rabbit -
Tested applications
Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
Immunogen
Rabbit IgG whole molecule
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution.
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ELISA |
Use at an assay dependent dilution.
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Immunomicroscopy |
Use at an assay dependent concentration.
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|
Dot blot |
Use at an assay dependent concentration.
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ICC/IF |
1/1000 - 1/5000.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent dilution. |
ELISA
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
1/1000 - 1/5000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab6828 has been referenced in 5 publications.
- Breuer T et al. Dexmedetomidine Impairs Diaphragm Function and Increases Oxidative Stress but Does Not Aggravate Diaphragmatic Atrophy in Mechanically Ventilated Rats. Anesthesiology 128:784-795 (2018). PubMed: 29346133
- Sugawa F et al. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile. EMBO J 34:1009-24 (2015). IHC-P ; Human . PubMed: 25750208
- Takahashi N et al. Epithelial TRPV1 Signaling Accelerates Gingival Epithelial Cell Proliferation. J Dent Res 93:1141-7 (2014). PubMed: 25266715
- Kawasaki K et al. Bmp signalling in filiform tongue papillae development. Arch Oral Biol 57:805-13 (2012). PubMed: 22186069
- Andrade E et al. cDNA microarray analysis of differentially expressed genes in penile tissue after treatment with tadalafil. BJU Int 101:508-12 (2008). PubMed: 18005212