Chicken Anti-Rabbit IgG H&L (FITC) (ab6825)
Key features and details
- Chicken Anti-Rabbit IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Chicken
- Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA
Related conjugates and formulations
Overview
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Product name
Chicken Anti-Rabbit IgG H&L (FITC)
See all IgG secondary antibodies -
Host species
Chicken -
Target species
Rabbit -
Tested applications
Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details -
Immunogen
Rabbit IgG whole molecule
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Immunomicroscopy |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent dilution.
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ICC/IF |
1/1000 - 1/5000.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ELISA |
1/10000 - 1/50000.
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Notes |
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Immunomicroscopy
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
ICC/IF
1/1000 - 1/5000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab6825 has been referenced in 4 publications.
- Druzhkova I et al. Expression of EMT-Related Genes in Hybrid E/M Colorectal Cancer Cells Determines Fibroblast Activation and Collagen Remodeling. Int J Mol Sci 21:N/A (2020). PubMed: 33143259
- Ford AJ et al. Fibroblasts stimulate macrophage migration in interconnected extracellular matrices through tunnel formation and fiber alignment. Biomaterials 209:88-102 (2019). PubMed: 31030083
- Brooks L et al. Fermentable carbohydrate stimulates FFAR2-dependent colonic PYY cell expansion to increase satiety. Mol Metab 6:48-60 (2017). PubMed: 28123937
- Celik S et al. Extracting a low-dimensional description of multiple gene expression datasets reveals a potential driver for tumor-associated stroma in ovarian cancer. Genome Med 8:66 (2016). PubMed: 27287041