Chicken Anti-Rabbit IgG H&L (TRITC) (ab6826)

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Overview

  • Product name
    Chicken Anti-Rabbit IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Host species
    Chicken
  • Target species
    Rabbit
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Rabbit IgG, whole molecule

  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • Conjugation notes
    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
  • Application notes
    suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

    • References

    This product has been referenced in:
    • Prozialeck WC  et al. Evaluation of cystatin C as an early biomarker of cadmium nephrotoxicity in the rat. Biometals 29:131-46 (2016). Read more (PubMed: 26715107) »
    See 1 Publication for this product

    Publishing research using ab6826? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    ORDER NUMBER 114090 DESCRIPTION OF THE PROBLEM weak Non-specific staining SAMPLE Hela PRIMARY ANTIBODY ab2893 DETECTION METHOD fluorescent microscoop POSITIVE AND NEGATIVE CONTROLS USED pos control radiation with UV during 45 min. neg control no primary antibody ANTIBODY STORAGE CONDITIONS refrigerator FIXATION OF SAMPLE methanol 10min at -20?C paraformaldehyde during 30 min at roomtemp PERMEABILIZATION STEP triton-X 100 ice cold for 15 min BLOCKING CONDITIONS 1h 1%BSA in PBS SECONDARY ANTIBODY ab6826 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 11 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? fixation time and method blocking time dilution different dilutions of primary and secondary antibody ADDITIONAL NOTES Purchase order number 4203309615 I have changed different steps in the protocol. Nothing worked!! I always got the same red background. It always coloured the entire cell!! Even in the neg control. as a last resort, I tried another sec antibody. this antibody gave the correct result.(compared with literature) I can say that the secondary antibody does noth work at all.

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    Answer

    I'm sorry to hear you are experiencing problems with ab6826, the fact that a "no primary" control gave you high background does indeed point towards a non-specificity of a damaged vial. Could you please clarify what dilution of this secondary antibody you have tried and how long you incubated it for? Thank you for providing this piece of information which will help me understand your problem better, I look forward to hearing from you to resolve this matter,

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    Question

    I know it says purified IgG, but has it been affinity purified so that it does not cross react with other species. The antibody will be mixed with donkey, goat and other secondary antibodies

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    Answer

    All the information avaliable for this product is stated on the datasheet. The datasheet reads "This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose". Therefore this antibody will be specific for Rabbit Immunoglobulin.

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    Question

    Hi, there, I am gonna do the immunostaining on the human cell lines, the primary antibody I have now was raised in rabbit, and I need a secondary anti-rabbit antibody conjugated with rhogamine, on your list, there are so many secondary antibodis fit my requirments, but raised in different animals (donkey #6799, goat #6718, sheep #6792, and chicken #6826), could you please tell me which one I should use if I want to have the lowest backgroung (crossreaction) from the secondary antibody on the human cells? Thanks a lot Helen

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    Answer

    There should be no difference between any of these secondaries; they will all work similarly well. However, if you wish to reduce background with the human cells then we suggest using secondaries that have been pre-adsorbed against human serum, thus, Goat polyclonal to rabbit IgG H&L (rhodamine) Pre-Adsorbed https://www.abcam.com/go.cfm?p=4&Ab=7087 and Donkey polyclonal to rabbit IgG H&L (rhodamine) Pre-Adsorbed https://www.abcam.com/go.cfm?p=4&Ab=7080 These should give you the lowest background.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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