Overview

  • Product name
    Chicken Anti-Rabbit IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Host species
    Chicken
  • Target species
    Rabbit
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Rabbit IgG, whole molecule

  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • Conjugation notes
    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
  • Application notes
    suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
  • References

    This product has been referenced in:
    • Prozialeck WC  et al. Evaluation of cystatin C as an early biomarker of cadmium nephrotoxicity in the rat. Biometals 29:131-46 (2016). Read more (PubMed: 26715107) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    I'm sorry to hear you are experiencing problems with ab6826, the fact that a "no primary" control gave you high background does indeed point towards a non-specificity of a damaged vial. Could you please clarify what dilution of this secondary antibody you have tried and how long you incubated it for? Thank you for providing this piece of information which will help me understand your problem better, I look forward to hearing from you to resolve this matter,

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    Answer

    All the information avaliable for this product is stated on the datasheet. The datasheet reads "This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose". Therefore this antibody will be specific for Rabbit Immunoglobulin.

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    Answer

    There should be no difference between any of these secondaries; they will all work similarly well. However, if you wish to reduce background with the human cells then we suggest using secondaries that have been pre-adsorbed against human serum, thus, Goat polyclonal to rabbit IgG H&L (rhodamine) Pre-Adsorbed https://www.abcam.com/go.cfm?p=4&Ab=7087 and Donkey polyclonal to rabbit IgG H&L (rhodamine) Pre-Adsorbed https://www.abcam.com/go.cfm?p=4&Ab=7080 These should give you the lowest background.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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