Overview

  • Product name

    Chicken SAA ELISA Kit
    See all Serum Amyloid A kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.094 ng/ml
  • Range

    0.375 ng/ml - 24 ng/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 0.375ng/ml - 24ng/ml

  • Assay time

    1h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Chicken
  • Product overview

    SAA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of SAA protein in chicken plasma and serum.

  • Notes

    In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP Conjugated Enzyme Antibody 1 x 150µl
    1X Diluent Concentration 1 x 60ml
    20X Wash Buffer Concentrate 1 x 50ml
    Anti-Chicken SAA ELISA Microplate 1 unit
    Chicken SAA Calibrator (Lyophilized) 1 vial
    Chromogen Substrate Solution 1 x 12ml
    Stop Solution 1 x 12ml
  • Research areas

  • Relevance

    Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
  • Alternative names

    • Amyloid fibrils
    • SAA 2
    • Serum amyloid A1 isoform 2
    • TP53I4
    • Amyloid fibril protein AA
    • Amyloid protein A
    • Fibrils
    • MGC111216
    • OC
    • OTTMUSP0000004557
    • PIG 4
    • PIG4
    • SAA
    • SAA 1
    • SAA_HUMAN
    • SAA1
    • SAA2
    • serum amyloid A
    • Serum amyloid A 1
    • Serum amyloid A protein precursor
    • Serum amyloid A1 isoform 1
    • Serum amyloid protein A(4-101)
    • Tumor protein p53 inducible protein 4
    see all

Applications

Our Abpromise guarantee covers the use of ab205081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab205081 Chicken SAA ELISA Kit.

Protocols

References

ab205081 has not yet been referenced specifically in any publications.

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