Overview

  • Product name
    Anti-Chikungunya Virus antibody [3585]
    See all Chikungunya Virus primary antibodies
  • Description
    Mouse monoclonal [3585] to Chikungunya Virus
  • Host species
    Mouse
  • Specificity
    ab155841 is specific to Chikungunya virus. No cross reactivity with other Alphaviruses (Negative vs. EEE, WEE, VEE and other Arboviruses). Membrane staining is observed in ICC/IF. ab155841 does not react with other members of the Alphavirus.
  • Tested applications
    Suitable for: ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Other species
  • Immunogen

    native viral lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    3585
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
ELISA 1/20 - 1/200.

Target

  • Relevance
    Chikungunya is caused by the chikungunya virus (CHIKV), an arthropod-borne virus (arbovirus). Chikungunya is a member of the Alphavirus genus in the family Togaviridae. There are 29 different types of alphaviruses that cause diseases in humans and other mammals. These species of arboviruses have been classified into 7 antigenic complexes: Barmah Forest (BF), Eastern equine encephalitis (EEE), Middelburg (MID), Ndumu (NDU), Semliki Forest (SF), Venezuelan equine encephalitis (VEE), and Western equine encephalitis (WEE).
  • Database links

    References

    ab155841 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer


    I can confirm that ab155841 was produced using the R80422 strain. It is known to be reactive with the EU14 strain and the CHIKV SV-0451/96 strain. I am sorry it has not been specifically epitope mapped or tested yet with other strains.

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    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    No
    Sample
    Mouse Cell (Chikungunya virus infected NIH/3T3 cells)
    Specification
    Chikungunya virus infected NIH/3T3 cells
    Preparation
    Cell harvesting/tissue preparation method: Cells (in suspension) were treated with virus attachment for 1 h at 4 degree centigrade
    Sample buffer: PBS with 2% FBS

    Dhiraj Acharya

    Verified customer

    Submitted Mar 03 2015

    Answer

    Thank you for contacting us.

    For ab155841, the cells were fized in acetone, so permeabilization was not required. For the other antibodies, the cells were permeabilized.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information, or want to enroll in our testing program.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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