ChIP Kit (ab500)
Key features and details
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
ChIP Kit
See all ChIP Kit kits -
Sample type
Adherent cells, Suspension cells -
Product overview
ChIP kit ab500 provides a protocol and reagents for running ChIP assays including:
- cell lysis and chromatin extraction
- chromatin shearing and DNA fragment length analysis
- immunoprecipitation and DNA purification
DNA produced using the kit can be analyzed using qPCR.
The kit has been validated for ChIP assays with mammalian samples.
Chinese protocol available. See protocols section below.
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Notes
This kit uses Protein A sepharose beads for antibody pulldown. See table below for Protein A and Protein G binding affinities with antibodies from commonly used species. For other species of antibody, consult the table in the protocol booklet.
Species raised in
Isotype Protein A binding affinity Protein G binding affinity Rabbit All isotypes +++ ++ Goat All isotypes - ++ Mouse
IgG1 + +++ IgG2a +++ +++ IgG2b ++ ++ IgG3 + + IgM Use anti-mouse IgM ChIP assay products and guides
Find more ChIP assay / chromatin immunoprecipitation resources and products, ChIP antibody products, and other ChIP assay kits and related reagents.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: ChIPmore details
Unsuitable for: ChIP-seq
Properties
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Storage instructions
Please refer to protocols. -
Storage buffer
Kit Constituents: Please see protocol.
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Components 1 kit 1.25M Glycine 1 x 10ml Buffer A 1 x 10ml Buffer B 1 x 30ml Buffer C 1 x 30ml Buffer D 1 x 3ml ChIP Buffer 5X 1 x 84ml DNA Purifying Slurry 1 x 4ml ab1791 - H3 antibody 1 x 25µg PCR-Grade Water 1 x 10.2ml Protease Inhibitors 1 tablet Protein A Beads Unblocked 1 x 960µl Proteinase K 1 x 40µl -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP |
Use at an assay dependent concentration.
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| Notes |
|---|
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ChIP
Use at an assay dependent concentration. |
Images
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ChiPLi Q., PLoS One 8(9). Fig 2c. doi: 10.1371/journal.pone.0075470. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Chromatin immunoprecipitation assay was performed to define the interaction of serum response factor (SRF) with the intragenic serum response element (SRE) regulatory motif in mouse using ab500 ChIP Kit.
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ChIP - ChIP Kit (ab500)Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 (tri methyl K9) antibody (ab8898). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 2 µg of ab8898 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of transcribed region.
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ChIP - ChIP Kit (ab500)Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 (tri methyl K4) antibody (ab12209). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 5 µg of ab12209 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci). Primers and probes are located in the first kb of the transcribed region.
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ChIP - ChIP Kit (ab500)Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 antibody (ab1791). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 2 µg of ab1791 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
Datasheets and documents
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SDS download
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Datasheet download
References (96)
ab500 has been referenced in 96 publications.
- Zhou S et al. Cannabinoid receptor type 2 promotes kidney fibrosis through orchestrating ß-catenin signaling. Kidney Int 99:364-381 (2021). PubMed: 33152447
- Zhong C et al. Remodeling cancer stemness by collagen/fibronectin via the AKT and CDC42 signaling pathway crosstalk in glioma. Theranostics 11:1991-2005 (2021). PubMed: 33408794
- Li Q et al. ELF3 activated by a superenhancer and an autoregulatory feedback loop is required for high-level HLA-C expression on extravillous trophoblasts. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33622787
- Shin JH et al. AHR Regulates NK Cell Migration via ASB2-Mediated Ubiquitination of Filamin A. Front Immunol 12:624284 (2021). PubMed: 33717133
- Shuai L et al. DOT1L Regulates Thermogenic Adipocyte Differentiation and Function via Modulating H3K79 Methylation. Diabetes 70:1317-1333 (2021). PubMed: 33795413