Product nameChIP Kit (Histones, ChIP-seq)
ChIP Kit (Histones, ChIP-seq) (ab270812) provides a robust ChIP protocol suitable for the investigation of histone modifications within chromatin from cells and tissues. The protocol involves protein-DNA cross-linking with formaldehyde, followed by cell lysis and fragmentation of the cross-linked chromatin. The subsequent immunoprecipitation of chromatin is performed with an antibody (user supplied) specific to a target histone modification. Protein A magnetic beads are used to isolate the protein-DNA complexes of interest. The immunoprecipitated DNA is eluted and purified using unique IPure magnetic beads. The eluted DNA is ideal for ChIP-seq library preparation. Additionally, the kit includes a negative (IgG) and a positive (H3K4me3) control antibody and primer pairs amplifying H3K4me3 positive and negative loci.
Cells (100,000 cells to 1,000,000 cells per IP)
Tissues (1.5 mg to 5 mg of tissue per IP)
Storage instructionsPlease refer to protocols.
Components 24 tests 10 tests 5% BSA (DNA free) 1 x 144µl 1 x 60µl 5x ChIP Buffer C1 1 x 2.2ml 1 x 950µl Buffer C 1 x 1.6ml 1 x 700µl Carrier 1 x 64µl 1 x 28µl ChIP-seq grade antibody H3K4me3 1 x 8µg 1 x 8µg ChIP-seq grade GAPDH TSS primer pair 1 x 96µl 1 x 42µl ChIP-seq grade Myoglobin exon 2 primer pair 1 x 96µl 1 x 42µl ChIP-seq grade water 1 x 7ml 1 x 3ml Protein A-coated magnetic beads 1 x 480µl 1 x 200µl Elution Buffer E1 1 x 3.4ml 1 x 1.5ml Elution Buffer E2 1 x 144µl 1 x 64µl Glycine 1 x 400µl 1 x 200µl Purification Beads 1 x 320µl 1 x 140µl Lysis Buffer L1 1 x 40ml 1 x 20ml Lysis Buffer L2 1 x 40ml 1 x 20ml Protease inhibitor cocktail 1 x 76µl 1 x 35µl Rabbit IgG 1 x 8µg 1 x 8µg Shearing Buffer S1 1 x 7.2ml 1 x 3.6ml Wash Buffer 1 w/o iso-propanol 1 x 2ml 1 x 700µl Wash Buffer 2 w/o iso-propanol 1 x 2ml 1 x 700µl Wash Buffer W1 1 x 8.4ml 1 x 3.5ml Wash Buffer W2 1 x 8.4ml 1 x 3.5ml Wash Buffer W3 1 x 8.4ml 1 x 3.5ml Wash Buffer W4 1 x 8.4ml 1 x 3.5ml
ab270812 has not yet been referenced specifically in any publications.