Product nameChIP Kit - Plants
See all ChIP Kit kits
Assay time6h 00m
Species reactivityReacts with: Plants
Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In plants, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.
Abcam's ChIP Kit - Plants (ab117137) offers an advantageous tool for identifying direct genome-wide associations between specific regulatory proteins and their target genes in plant cells within 6 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing and southern blot, as well as DNA microarray.
This kit is designed for 24 or 48 ChIP reactions, not for 24 or 48 samples. The standard protocol of the kit allows for performing 8 reactions with one sample. For testing more samples, the amount of each sample should be reduced. The amount of each reagent used for chromatin preparation should be also proportionally reduced.
Tested applicationsSuitable for: ChIPmore details
Storage instructionsPlease refer to protocols.
Components 24 tests 48 tests 100X Protease Inhibitor Cocktail 1 x 25µl 1 x 50µl 5X Lysis Buffer I 1 x 12ml 1 x 24ml 8-Well Assay Strips (with Frame) 3 units 6 units 8-Well Strip Caps 3 units 6 units Antibody Buffer 1 x 15ml 1 x 30ml Anti-H3K9me2 (1 mg/mL) 1 x 5µl 1 x 8µl Binding Buffer 1 x 5ml 1 x 8ml ChIP Dilution Buffer 1 x 2ml 1 x 6ml DNA Release Buffer 1 x 2ml 2 x 2ml Elution Buffer 1 x 0.6ml 1 x 1.2ml F-Collection Tube 30 units 50 units F-Spin Column 30 units 50 units Lysis Buffer II 1 x 3ml 1 x 6ml Lysis Buffer III 1 x 2ml 1 x 4ml Lysis Buffer IV 1 x 1.5ml 1 x 5ml Normal Mouse IgG (1 mg/mL) 1 x 10µl 1 x 10µl Proteinase K (10 mg/mL) 1 x 25µl 1 x 50µl Reverse Buffer 1 x 2ml 2 x 2ml Wash Buffer 1 x 28ml 2 x 28ml
Our Abpromise guarantee covers the use of ab117137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
ab117137 has been referenced in 3 publications.
- Hufnagel B et al. High-quality genome sequence of white lupin provides insight into soil exploration and seed quality. Nat Commun 11:492 (2020). PubMed: 31980615
- Shi C et al. Maternal control of suspensor programmed cell death via gibberellin signaling. Nat Commun 10:3484 (2019). PubMed: 31375676
- Baker K et al. Chromatin state analysis of the barley epigenome reveals a higher order structure defined by H3K27me1 and H3K27me3 abundance. Plant J N/A:N/A (2015). PubMed: 26255869