Product nameChIP Kit (Transcription factors, ChIP-seq)
ChIP Kit (Transcription factors, ChIP-seq) (ab270813) is a highly validated solution for robust transcription factor and other non-histone proteins ChIP-seq results and contains everything you need for start-to-finish ChIP prior to Next-Generation Sequencing. This complete solution contains all buffers and reagents for cell lysis, chromatin shearing, immunoprecipitation, and DNA purification. This kit contains positive and negative control antibodies (CTCF and IgG, respectively) as well as positive and negative control PCR primers pairs (H19 and Myoglobin exon 2, respectively).
Cells (4,000,000 cells per IP)
Tissues (30 mg of tissue per IP)
Storage instructionsPlease refer to protocols.
Components 24 tests 10 tests 5% BSA (DNA free) 1 x 800µl 1 x 380µl 5x ChIP Buffer C1b 1 x 6.9ml 1 x 3.4ml Buffer C 1 x 1.6ml 1 x 700µl Carrier 1 x 72µl 1 x 32µl ChIP-seq grade CTCF antibody 1 x 8µg 1 x 4µg ChIP-seq grade H19 imprinting control region primer pair (human) 1 x 96µl 1 x 42µl ChIP-seq grade Myoglobin exon 2 primer pair (human) 1 x 96µl 1 x 42µl ChIP-seq grade water 1 x 26.6ml 1 x 14ml Elution Buffer E1 1 x 3.4ml 1 x 1.5ml Elution-buffer E2 1 x 144µl 1 x 64µl Fixation buffer 1 x 8ml 1 x 4ml Glycine 1 x 8.8ml 1 x 4.4ml Lysis Buffer L1b 1 x 100ml 1 x 50ml Lysis Buffer L2 1 x 60ml 1 x 30ml Protease inhibitor cocktail 1 x 80µl 1 x 38µl Protein A-coated magnetic beads 1 x 720µl 1 x 300µl Purification Beads 1 x 400µl 1 x 180µl Rabbit IgG 1 x 8µg 1 x 4µg Shearing Buffer S1b 1 x 6.7ml 1 x 3.4ml Wash buffer 1 w/o iso-propanol 1 x 2ml 1 x 900µl Wash buffer 2 w/o iso-propanol 1 x 2ml 1 x 900µl Wash buffer W1 1 x 8.4ml 1 x 3.5ml Wash buffer W2 1 x 8.4ml 1 x 3.5ml Wash buffer W3 1 x 8.4ml 1 x 3.5ml Wash buffer W4 1 x 8.4ml 1 x 3.5ml
ab270813 has not yet been referenced specifically in any publications.