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    chip-kit-transcription-factors-chip-seq-ab270813.pdf

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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription
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ChIP Kit (Transcription factors, ChIP-seq) (ab270813)

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ChIP-sequencing - Anti-RNA polII CTD S2P antibody [EPR18855-87] - ChIP Grade (ab238146)
  • ChIP-sequencing - Anti-SP1 antibody [EPR22648-50] - ChIP Grade (ab231778)
  • ChIP-sequencing - Anti-TBP antibody [EPR21954] - ChIP Grade (ab220788)
  • ChIP-sequencing - Anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408)

Overview

  • Product name

    ChIP Kit (Transcription factors, ChIP-seq)
  • Product overview

    ChIP Kit (Transcription factors, ChIP-seq) (ab270813) is a highly validated solution for robust transcription factor and other non-histone proteins ChIP-seq results and contains everything you need for start-to-finish ChIP prior to Next-Generation Sequencing. This complete solution contains all buffers and reagents for cell lysis, chromatin shearing, immunoprecipitation, and DNA purification. This kit contains positive and negative control antibodies (CTCF and IgG, respectively) as well as positive and negative control PCR primers pairs (H19 and Myoglobin exon 2, respectively).


    Sample size:


    Cells (4,000,000 cells per IP)


    Tissues (30 mg of tissue per IP)

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 24 tests 10 tests
    5% BSA (DNA free) 1 x 800µl 1 x 380µl
    5x ChIP Buffer C1b 1 x 6.9ml 1 x 3.4ml
    Buffer C 1 x 1.6ml 1 x 700µl
    Carrier 1 x 72µl 1 x 32µl
    ChIP-seq grade CTCF antibody 1 x 8µg 1 x 4µg
    ChIP-seq grade H19 imprinting control region primer pair (human) 1 x 96µl 1 x 42µl
    ChIP-seq grade Myoglobin exon 2 primer pair (human) 1 x 96µl 1 x 42µl
    ChIP-seq grade water 1 x 26.6ml 1 x 14ml
    Elution Buffer E1 1 x 3.4ml 1 x 1.5ml
    Elution-buffer E2 1 x 144µl 1 x 64µl
    Fixation buffer 1 x 8ml 1 x 4ml
    Glycine 1 x 8.8ml 1 x 4.4ml
    Lysis Buffer L1b 1 x 100ml 1 x 50ml
    Lysis Buffer L2 1 x 60ml 1 x 30ml
    Protease inhibitor cocktail 1 x 80µl 1 x 38µl
    Protein A-coated magnetic beads 1 x 720µl 1 x 300µl
    Purification Beads 1 x 400µl 1 x 180µl
    Rabbit IgG 1 x 8µg 1 x 4µg
    Shearing Buffer S1b 1 x 6.7ml 1 x 3.4ml
    Wash buffer 1 w/o iso-propanol 1 x 2ml 1 x 900µl
    Wash buffer 2 w/o iso-propanol 1 x 2ml 1 x 900µl
    Wash buffer W1 1 x 8.4ml 1 x 3.5ml
    Wash buffer W2 1 x 8.4ml 1 x 3.5ml
    Wash buffer W3 1 x 8.4ml 1 x 3.5ml
    Wash buffer W4 1 x 8.4ml 1 x 3.5ml
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • TFIIB
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Polymerase
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • TAFs
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Nuclear
    • Isotype/Loading Controls
    • Loading Controls
    • TBP - Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • RNA polymerase
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Preinitiation complex
    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • Methylated DNA Associated
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial Biogenesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Molecular processes
    • Mitochondrial transcription
  • Cellular localization

    RNA polymerase II CTD repeat YSPTSPS: Nucleus. TATA binding protein TBP: Nucleus. SP1: Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location. CTCF: Nucleus > nucleoplasm. Chromosome. Chromosome > centromere. May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.

Images

  • ChIP-sequencing - Anti-RNA polII CTD S2P antibody [EPR18855-87] - ChIP Grade (ab238146)
    ChIP-sequencing - Anti-RNA polII CTD S2P antibody [EPR18855-87] - ChIP Grade (ab238146)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab238146. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).

  • ChIP-sequencing - Anti-SP1 antibody [EPR22648-50] - ChIP Grade (ab231778)
    ChIP-sequencing - Anti-SP1 antibody [EPR22648-50] - ChIP Grade (ab231778)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab231778. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).

  • ChIP-sequencing - Anti-TBP antibody [EPR21954] - ChIP Grade (ab220788)
    ChIP-sequencing - Anti-TBP antibody [EPR21954] - ChIP Grade (ab220788)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab220788. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813). 

  • ChIP-sequencing - Anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408)
    ChIP-sequencing - Anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab188408. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).

Protocols

  • Protocol Booklet
  • Protocol Booklet
  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

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References (0)

Publishing research using ab270813? Please let us know so that we can cite the reference in this datasheet.

ab270813 has not yet been referenced specifically in any publications.

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