ChIP Kit (Transcription factors, ChIP-seq) (ab270813)

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ChIP-sequencing - Anti-RNA polII CTD S2P antibody [EPR18855-87] - ChIP Grade (ab238146)
  • ChIP-sequencing - Anti-SP1 antibody [EPR22648-50] - ChIP Grade (ab231778)
  • ChIP-sequencing - Anti-TBP antibody [EPR21954] - ChIP Grade (ab220788)
  • ChIP-sequencing - Anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408)

Overview

  • Product name

    ChIP Kit (Transcription factors, ChIP-seq)

  • Product overview

    ChIP Kit (Transcription factors, ChIP-seq) (ab270813) is a highly validated solution for robust transcription factor and other non-histone proteins ChIP-seq results and contains everything you need for start-to-finish ChIP prior to Next-Generation Sequencing. This complete solution contains all buffers and reagents for cell lysis, chromatin shearing, immunoprecipitation, and DNA purification. This kit contains positive and negative control antibodies (CTCF and IgG, respectively) as well as positive and negative control PCR primers pairs (H19 and Myoglobin exon 2, respectively).


    Sample size:


    Cells (4,000,000 cells per IP)


    Tissues (30 mg of tissue per IP)

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties

Images

  • ChIP-sequencing - Anti-RNA polII CTD S2P antibody [EPR18855-87] - ChIP Grade (ab238146)
    ChIP-sequencing - Anti-RNA polII CTD S2P antibody [EPR18855-87] - ChIP Grade (ab238146)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab238146. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).

  • ChIP-sequencing - Anti-SP1 antibody [EPR22648-50] - ChIP Grade (ab231778)
    ChIP-sequencing - Anti-SP1 antibody [EPR22648-50] - ChIP Grade (ab231778)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab231778. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).

  • ChIP-sequencing - Anti-TBP antibody [EPR21954] - ChIP Grade (ab220788)
    ChIP-sequencing - Anti-TBP antibody [EPR21954] - ChIP Grade (ab220788)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab220788. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813). 

  • ChIP-sequencing - Anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408)
    ChIP-sequencing - Anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab188408. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).

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ab270813 has not yet been referenced specifically in any publications.

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