For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    chip-seq-high-sensitivity-kit-ab185908.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Assays & Kits ChIP Related Products
Share by email

ChIP-Seq High Sensitivity Kit (ab185908)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Size distribution of library fragments.
  • High sensitive ChIP
  • Sample results

Key features and details

  • Assay time: 7 hr
  • Sample type: Adherent cells, Suspension cells, Tissue

You may also be interested in

Preparation
Extraction Enhancer Buffer 50X (ab193971)
Kit
Product image
Chromatin Extraction Kit - Flexible Format (ab223876)
 
Product image
GAPDH positive control ChIP primer pair (ab267832)

View more associated products

Overview

  • Product name

    ChIP-Seq High Sensitivity Kit
    See all ChIP Kit kits
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Assay time

    7h 00m
  • Product overview

    ab185908 is a complete set of reagents required for carrying out a successful ChIP-Seq starting from mammalian cells or tissues. The kit is designed to selectively enrich a chromatin fraction containing specific DNA sequences from various species, particularly mammals, and to prepare a ChIP-Seq library for next generation sequencing using Illumina® platforms such as Illumina® Genome Analyzer II, HiSeq and MiSeq systems. The optimized protocol and components of the kit allow capture of low abundance protein/DNA complexes with minimized non-specific background levels and the ability to construct both non-barcoded (singleplexed) and barcoded (multiplexed) ChIP-Seq libraries quickly with reduced bias.

    Starting Materials:
    Starting materials can include various tissue or cell samples such as culture cells from a flask or plate, fresh and frozen tissues, etc.

    Input Amount of Tissue/Cells:

    In general, the amount of cells and tissues for each reaction can be 1 x 105 to 1 x 106 and 5 mg to 50 mg, respectively. For optimal preparation, the input amount should be 4 to 5 x 105cells or 20 to 30 mg tissues so that the amount of DNA enriched from the ChIP reaction can range from at least 1 ng to 100 ng.

    Illumina® is a registered trademark of Illumina, Inc.

  • Notes

    Protein-DNA interaction plays a critical role for cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanisms of protein-DNA interaction on a genome-wide scale is important for understanding cellular processes. Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) offers an advantageous tool for studying genome-wide protein-DNA interactions. It allows for detection that a specific protein binds to specific sequences in living cells. In particular, ChIP antibodies targeted against various transcriptional factors (TF) for genome-wide transcription factor binding site analysis by Chip-Seq is in high demand. Such analysis requires that ChIPed DNA contain minimal background for reliably identifying true TF-enriched regions. Currently used ChIP-Seq methods play an important role in identifying genome-wide protein-DNA interaction.  The ChIP-Seq High Sensitivity Kit combines microplate-based ultra ChIP and high sensitive DNA library construction technologies.

  • Tested applications

    Suitable for: ChIP-sequencingmore details

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 12 tests 24 tests
    1000X Protease Inhibitor Cocktail 1 x 15µl 1 x 30µl
    10X End Polishing Buffer 1 x 30µl 1 x 60µl
    2X HiFi PCR Master Mix 1 x 160µl 1 x 320µl
    2X Ligation Buffer 1 x 250µl 1 x 500µl
    8-Well Assay Strips (with Frame) 1 x 2 units 1 x 4 units
    8-Well Strip Caps 1 x 2 units 1 x 4 units
    Adaptors (50 μM) 1 x 15µl 1 x 30µl
    Adhesive 8-Well Strip Film 1 x 4 units 1 x 8 units
    Antibody Buffer 1 x 1ml 1 x 2ml
    Anti-RNA Polymerase II 1 x 5µl 1 x 10µl
    Blocker Solution 1 x 1ml 1 x 2ml
    ChIP Buffer 1 x 6ml 1 x 12ml
    DNA Binding Solution 1 x 7ml 1 x 14ml
    DNA Elution Buffer 1 x 0.5ml 1 x 1ml
    DNA Release Buffer 1 x 7ml 1 x 14ml
    Elution Buffer 1 x 1ml 1 x 2ml
    End Polishing Enhancer 1 x 13µl 1 x 26µl
    End Polishing Enzyme Mix 1 x 13µl 1 x 26µl
    Enrichment Enhancer 1 x 25µl 1 x 50µl
    F-Collection Tube 1 x 15 units 1 x 30 units
    F-Spin Column 1 x 15 units 1 x 30 units
    GAPDH Primer - Forward (20 µM) 1 x 5µl 1 x 10µl
    GAPDH Primer - Reverse (20 µM) 1 x 5µl 1 x 10µl
    Lysis Buffer 1 x 7ml 1 x 14ml
    MQ Binding Beads 1 x 1.6ml 2 x 1.6ml
    Non-Immune IgG (1 mg/ml) 1 x 5µl 1 x 10µl
    Primer I (10 μM) 1 x 15µl 1 x 30µl
    Primer U (10 μM) 1 x 15µl 1 x 30µl
    Proteinase K (10 mg/mL) 1 x 30µl 1 x 60µl
    Rnase A 1 x 15µl 1 x 30µl
    T4 DNA Ligase 1 x 15µl 1 x 30µl
    Wash Buffer 1 x 12ml 1 x 25ml
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Assays & Kits
    • ChIP Related Products
    • Kits/ Lysates/ Other
    • Kits
    • Epigenetic kits
    • ChIP Kits
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP kits

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP-sequencing
Use at an assay dependent concentration.
Notes
ChIP-sequencing
Use at an assay dependent concentration.

Images

  • Size distribution of library fragments.
    Size distribution of library fragments.

    Ten nanograms of DNA was ChIPed by RNA polymerase II enrichment and used for DNA library preparation.

  • High sensitive ChIP
    High sensitive ChIP

    The sheared chromatin isolated from different number of MBD-231 cells was used for ChIP-qPCR analysis of RNA polymerase II enrichment in GAPDH promoters.

  • Sample results
    Sample results

    A ChIP-sequencing library was prepared using ChIP-Seq High Sensitivity Kit (ab185908) from rat heart chromatin and polyclonal antibodies against H3K4me3, H3(K9/14)ac and, H3K27me3. Sequencing was carried out on an Illumina HiSeq2500. Bioinformatics analysis of ChIP-seq is performed utilizing Bowtie and MACS. 

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab185908? Please let us know so that we can cite the reference in this datasheet.

ab185908 has been referenced in 1 publication.

  • Zhang Y  et al. LncRNA-CCDC144NL-AS1 Promotes the Development of Hepatocellular Carcinoma by Inducing WDR5 Expression via Sponging miR-940. J Hepatocell Carcinoma 8:333-348 (2021). PubMed: 33977095

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab185908.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.