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    chir99021-gsk3-inhibitor-ab120890.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Cdks
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CHIR99021, GSK3 inhibitor (ab120890)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (7)

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Chemical Structure - CHIR99021, GSK3 inhibitor (ab120890)

    Key features and details

    • Potent, highly selective GSK3 inhibitor
    • CAS Number: 252917-06-9
    • Purity: > 98%
    • Soluble in DMSO to 100 mM
    • Form / State: Solid
    • Source: Synthetic

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    Overview

    • Product name

      CHIR99021, GSK3 inhibitor
    • Description

      Potent, highly selective GSK3 inhibitor
    • Biological description

      Potent, highly selective aminopyrimidine GSK3 inhibitor (IC50 values are 6.7 and 10 nM at GSK3β and GSK3α, respectively). Exhibits >500-fold selectivity for GSK3 against 20 closely related protein kinases and >800-fold selectivity for 23 additional enzymes and 22 receptors. Promotes self-renewal of mouse and human ESC due to β-catenin and c-Myc protein level stabilization. Commonly used in multiple stages of organoid generation.

    • Purity

      > 98%
    • CAS Number

      252917-06-9
    • Chemical structure

      Chemical Structure

    Properties

    • Chemical name

      6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile
    • Molecular weight

      465.34
    • Molecular formula

      C22H18Cl2N8
    • PubChem identifier

      9956119
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
    • Solubility overview

      Soluble in DMSO to 100 mM
    • Handling

      Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

      Toxic, refer to SDS for further information.

      Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

    • SMILES

      N#CC(C=N1)=CC=C1NCCNC2=NC=C(C3=NC(C)=CN3)C(C4=CC=C(Cl)C=C4Cl)=N2
    • Source

      Synthetic

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    Images

    • Chemical Structure - CHIR99021, GSK3 inhibitor (ab120890)
      Chemical Structure - CHIR99021, GSK3 inhibitor (ab120890)
      2D chemical structure image of ab120890, CHIR99021, GSK3 inhibitor

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab120890? Please let us know so that we can cite the reference in this datasheet.

    ab120890 has been referenced in 7 publications.

    • Saxena M  et al. A Pygopus 2-Histone Interaction Is Critical for Cancer Cell Dedifferentiation and Progression in Malignant Breast Cancer. Cancer Res 80:3631-3648 (2020). PubMed: 32586983
    • Elling U  et al. Derivation and maintenance of mouse haploid embryonic stem cells. Nat Protoc 14:1991-2014 (2019). PubMed: 31160788
    • Balmus G  et al. ATM orchestrates the DNA-damage response to counter toxic non-homologous end-joining at broken replication forks. Nat Commun 10:87 (2019). PubMed: 30622252
    • Jeziorowska D  et al. Differential Sarcomere and Electrophysiological Maturation of Human iPSC-Derived Cardiac Myocytes in Monolayer vs. Aggregation-Based Differentiation Protocols. Int J Mol Sci 18:N/A (2017). PubMed: 28587156
    • Sato H  et al. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells. Sci Rep 6:31063 (2016). PubMed: 27507707
    • Fagnocchi L  et al. A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity. Nat Commun 7:11903 (2016). PubMed: 27301576
    • Song J & Millman JR Economic 3D-printing approach for transplantation of human stem cell-derived ß-like cells. Biofabrication 9:015002 (2016). PubMed: 27906687

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    how can this compound be qantified by spectroscopy?
    ex/em wavelength?
    by HPLC which wavelength is being used to detect it?

    Read More

    Abcam community

    Verified customer

    Asked on May 17 2013

    Answer


    The lab let me know that we actually qualify CHIR99021 by TLC, MS and UV (not by HPLC). The absorption is measured by UV λ max: 279 nm.

    Read More

    Abcam Scientific Support

    Answered on May 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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