Product nameAnti-Chitinase 3 like protein 3 antibody
See all Chitinase 3 like protein 3 primary antibodies
DescriptionRabbit polyclonal to Chitinase 3 like protein 3
Tested applicationsSuitable for: WB, ICC/IF, IHC-FoFrmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Human Chitinase 3 like protein 3 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Mouse bone marrow and spleen tissue lysates. It also gave a positive signal in RAW 246.7 cells in IF/ICC.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 28754163|
RelevanceChitinase-3-like protein 3 (Ym1) is a lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine; binding to oligomeric saccharides is much stronger. It also binds chitin and heparin. This protein has been implicated in inflammation and allergy.
- YM1 antibody
ICC/IF image of ab93034 stained RAW 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93034 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Chitinase 3 like protein 3 antibody (ab93034) at 1 µg/ml
Lane 1 : Mouse Bone Marrow Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 26 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
This product has been referenced in:
- Uhde AK et al. Intact interleukin-10 receptor signaling protects from hippocampal damage elicited by experimental neurotropic virus infection of SJL mice. Sci Rep 8:6106 (2018). Read more (PubMed: 29666403) »
- Wang X et al. Macrophage inducible nitric oxide synthase circulates inflammation and promotes lung carcinogenesis. Cell Death Discov 4:46 (2018). Read more (PubMed: 29844930) »