Recombinant

Recombinant Anti-Chitinase 3 like protein 3 antibody [EPR21248] (ab230610)

Overview

  • Product name

    Anti-Chitinase 3 like protein 3 antibody [EPR21248]
    See all Chitinase 3 like protein 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR21248] to Chitinase 3 like protein 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Ms spleen FFPE and Ms Lung FFPE (Ms skeletal muscle FFPE was used as a negative tissue)
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/Rabbit Fc).

Properties

Applications

Our Abpromise guarantee covers the use of ab230610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.01 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance

    Chitinase-3-like protein 3 (Ym1) is a lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine; binding to oligomeric saccharides is much stronger. It also binds chitin and heparin. This protein has been implicated in inflammation and allergy.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • YM1 antibody

Images

  • IHC image of Chitinase 3 like protein 3 staining in mouse lung formalin fixed paraffin embedded tissue section, performed on a Leica BONDTM system using the standard protocol F (with no post primary). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab230610, 0.01µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of Chitinase 3 like protein 3 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BONDTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab230610, 0.01µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab230610 has not yet been referenced specifically in any publications.

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