Overview

  • Product name

  • Description

    Rabbit polyclonal to Chk1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within the internal sequence amino acids 259-351 of Human Chk1 (Uniprot ID O14757).

  • Positive control

    • 293T nuclear extract, A431, H1299, Molt-4, Raji, HeLa cells, SCM-1 xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab137400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • C85740 antibody
    • Cell cycle checkpoint kinase antibody
    • Checkpoint , S. pombe, homolog of, 1 antibody
    • Checkpoint kinase 1 antibody
    • Checkpoint kinase 1 homolog (S. pombe) antibody
    • CHEK 1 antibody
    • Chek1 antibody
    • Chk 1 antibody
    • Chk1 antibody
    • CHK1 checkpoint homolog (S. pombe) antibody
    • CHK1_HUMAN antibody
    • EC 2.7.11.1 antibody
    • rad27 antibody
    • Serine/threonine protein kinase Chk1 antibody
    • Serine/threonine-protein kinase CHK1 antibody
    • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
    see all

Images

  • Anti-Chk1 antibody (ab137400) at 1/1000 dilution + 293T nuclear extract at 20 µg

    Predicted band size: 54 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelled with ab137400 at 1/200 dilution. Costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded SCM-1 xenograft tissue labelled with ab137400 at 1/100 dilution.

References

ab137400 has not yet been referenced specifically in any publications.

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