Product nameAnti-Chk1 antibody [E250]
See all Chk1 primary antibodies
DescriptionRabbit monoclonal [E250] to Chk1
Specificityab32531 recognises Chk1.
Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IPmore details
Unsuitable for: IHC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Chk1 aa 1-100 (N terminal). The exact sequence is proprietary.
- K562 cell lysate
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).|
|Flow Cyt||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
- Information by UniProt
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
Anti-Chk1 antibody [E250] (ab32531) at 1/1000 dilution + K562 cell lysate
Predicted band size: 54 kDa
Observed band size: 54 kDa
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling Chk1 with Purified ab32531 at 1/250 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling Chk1 with unpurified ab32531 at 1/200 dilution (1µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This product has been referenced in:
- Peng J et al. RECQL5 plays an essential role in maintaining genome stability and viability of triple-negative breast cancer cells. Cancer Med 8:4743-4752 (2019). Read more (PubMed: 31231988) »
- Liu H et al. The Deubiquitylating Enzyme USP4 Cooperates with CtIP in DNA Double-Strand Break End Resection. Cell Rep 13:93-107 (2015). Read more (PubMed: 26387952) »