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  1. Link

    chk1-antibody-ep691y-ab40866.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response Chk1 / Chk2
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RecombinantRabMAb

Recombinant Anti-Chk1 antibody [EP691Y] (ab40866)

  • Datasheet
Reviews (6)Q&A (1)References (23)

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Western blot - Anti-Chk1 antibody [EP691Y] (ab40866)
  • Immunocytochemistry/ Immunofluorescence - Anti-Chk1 antibody [EP691Y] (ab40866)
  • Flow Cytometry (Intracellular) - Anti-Chk1 antibody [EP691Y] (ab40866)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk1 antibody [EP691Y] (ab40866)
  • Anti-Chk1 antibody [EP691Y] (ab40866)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP691Y] to Chk1
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-Chk1 antibody [EP691Y]
    See all Chk1 primary antibodies
  • Description

    Rabbit monoclonal [EP691Y] to Chk1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Chk1 aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa cells lysate and human breast carcinoma.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP691Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Chk1 / Chk2
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Chk1 antibody [EP691Y] (ab196308)
    • Alexa Fluor® 647 Anti-Chk1 antibody [EP691Y] (ab196520)
    • Anti-Chk1 antibody [EP691Y] - BSA and Azide free (ab210964)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant human Chk1 protein (ab69918)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100 - 1/250.
WB (5)
1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P (1)
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100 - 1/250.
WB
1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Target information above from: UniProt accession O14757 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1111 Human
    • Omim: 603078 Human
    • SwissProt: O14757 Human
    • Unigene: 24529 Human
    • Alternative names

      • C85740 antibody
      • Cell cycle checkpoint kinase antibody
      • Checkpoint , S. pombe, homolog of, 1 antibody
      • Checkpoint kinase 1 antibody
      • Checkpoint kinase 1 homolog (S. pombe) antibody
      • CHEK 1 antibody
      • Chek1 antibody
      • Chk 1 antibody
      • Chk1 antibody
      • CHK1 checkpoint homolog (S. pombe) antibody
      • CHK1_HUMAN antibody
      • EC 2.7.11.1 antibody
      • rad27 antibody
      • Serine/threonine protein kinase Chk1 antibody
      • Serine/threonine-protein kinase CHK1 antibody
      • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
      see all

    Images

    • Western blot - Anti-Chk1 antibody [EP691Y] (ab40866)
      Western blot - Anti-Chk1 antibody [EP691Y] (ab40866)
      Anti-Chk1 antibody [EP691Y] (ab40866) at 1/10000 dilution + HeLa cell lysate at 10 µg

      Predicted band size: 54 kDa
      Observed band size: 54 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Chk1 antibody [EP691Y] (ab40866)
      Immunocytochemistry/ Immunofluorescence - Anti-Chk1 antibody [EP691Y] (ab40866)

      Immunofluorescence staining of MCF7 cells with purified ab40866 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    • Flow Cytometry (Intracellular) - Anti-Chk1 antibody [EP691Y] (ab40866)
      Flow Cytometry (Intracellular) - Anti-Chk1 antibody [EP691Y] (ab40866)

      Overlay histogram showing HeLa cells stained with ab40866 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40866, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk1 antibody [EP691Y] (ab40866)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk1 antibody [EP691Y] (ab40866)

      Immunohistochemical analysis of Chk1 expression in paraffin-embedded human breast carcinoma using ab40866 at a 1:250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Chk1 antibody [EP691Y] (ab40866)
      Anti-Chk1 antibody [EP691Y] (ab40866)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (23)

    Publishing research using ab40866? Please let us know so that we can cite the reference in this datasheet.

    ab40866 has been referenced in 23 publications.

    • Chou HC  et al. The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structure. Elife 10:N/A (2021). PubMed: 33522487
    • Gong D  et al. Silencing Long Non-coding RNA LINC01224 Inhibits Hepatocellular Carcinoma Progression via MicroRNA-330-5p-Induced Inhibition of CHEK1. Mol Ther Nucleic Acids 19:482-497 (2020). PubMed: 31902747
    • Ding J  et al. lncRNA CCAT2 Enhanced Resistance of Glioma Cells Against Chemodrugs by Disturbing the Normal Function of miR-424. Onco Targets Ther 13:1431-1445 (2020). PubMed: 32110042
    • Xu W  et al. The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer. Int J Biol Sci 16:1388-1402 (2020). PubMed: 32210727
    • Ren S  et al. ATM-mediated DNA double-strand break response facilitated oncolytic Newcastle disease virus replication and promoted syncytium formation in tumor cells. PLoS Pathog 16:e1008514 (2020). PubMed: 32479542
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Chk1 antibody [EP691Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH6.0
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 03 2015

    Western blot abreview for Anti-Chk1 antibody [EP691Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (HCT116)
    Specification
    HCT116
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 17 2013

    Western blot abreview for Anti-Chk1 antibody [EP691Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human colon cancer cells)
    Loading amount
    25 µg
    Specification
    human colon cancer cells
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 22 2013

    Western blot abreview for Anti-Chk1 antibody [EP691Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human mammary cell)
    Loading amount
    50000 cells
    Specification
    Human mammary cell
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 11 2012

    Western blot abreview for Anti-Chk1 antibody [EP691Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human breast adenocarcinoma cell line (MCF7))
    Loading amount
    35 µg
    Specification
    human breast adenocarcinoma cell line (MCF7)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 14 2011

    Western blot abreview for Anti-Chk1 antibody [EP691Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Colon cell line)
    Loading amount
    20 µg
    Specification
    Colon cell line
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 09 2011

    Question

    I am writing to you to ask some information's about the Chk1 antibodies commercialized by Abcam. I work with S.pombe and as I understand from the website there is no Ab against S. pombe chk1? Do you have any information if any of the chk1 ab you have will react with S.pombe chk1 in order to perform western blots as well as immonufluorescence?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 06 2007

    Answer

    Thank you for your enquiry. Unfortunately, based on the sequence alignment, this anti-chk1 will not work for s. pombe samples. I hope this information helps. Please do not hesitate to contact us if you need anything further.

    Read More

    Abcam Scientific Support

    Answered on Feb 06 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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