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Rabbit polyclonal to Chk1 (phospho S286)
ab193234 detects endogenous level of Chk1 only when phosphorylated at serine 286.
Synthetic peptide within Human Chk1 (phospho S286) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Peptide sequence around phosphorylation site of serine 286 (S-E-S(p)-P-S) .
Database link: O14757
K562 cell lysate, treated with Na3VO4 (Sodium orthovanadate).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituents: 0.87% Sodium chloride, 50% Glycerol, 49% PBS PBS (without Mg2 +and Ca 2+)
Concentration information loading...
Immunogen affinity purified
ab193234 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 54 kDa.
Information by UniProt
Cell cycle checkpoint kinase antibody
Checkpoint , S. pombe, homolog of, 1 antibody
Western blot - Anti-Chk1 (phospho S286) antibody (ab193234)
All lanes : Anti-Chk1 (phospho S286) antibody (ab193234) at 1/500 dilution Lane 1 : K562 cell lysate, treated with Na3VO4 Lane 2 : K562 cell lysate, treated with Na3VO4 with antigen-specific peptide Predicted band size: 54 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"