Recombinant Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR915] to Chk1 (phospho S296)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Chk1 (phospho S296) antibody [EPR915]
See all Chk1 primary antibodies -
Description
Rabbit monoclonal [EPR915] to Chk1 (phospho S296) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 283T cell lysate treated with UV and AP; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.1% BSA, 9.85% Tris glycine -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR915 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
For unpurified use at 1/1000 - 1/2000 |
Notes |
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WB
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). For unpurified use at 1/1000 - 1/2000 |
Target
- Information by UniProt
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Database links
- Entrez Gene: 1111 Human
- Omim: 603078 Human
- SwissProt: O14757 Human
- Unigene: 24529 Human
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Alternative names
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
see all
Images
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All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1500 dilution
Lane 1 : Untreated 293 cell lysate
Lane 2 : Treated with Calyculin A 293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution
Predicted band size: 54 kDa -
All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1000 dilution (Unpurified)
Lane 1 : Untreated 293 cell Lysate
Lane 2 : 293 cell lysate, treated with Calyculin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab79758 has been referenced in 20 publications.
- Wang YC et al. Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination. Cell Rep 42:112296 (2023). PubMed: 36961817
- Luo Q et al. Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity. Cell Biol Toxicol N/A:N/A (2022). PubMed: 35142956
- Wang L et al. Long Noncoding RNA Solute Carrier Family 25 Member 21 Antisense RNA 1 Inhibits Cell Malignant Behaviors and Enhances Radiosensitivity of Gastric Cancer Cells by Upregulating Synuclein Gamma Expression. Tohoku J Exp Med 257:225-239 (2022). PubMed: 35444105
- Redwood AB et al. The cytosolic iron-sulfur cluster assembly (CIA) pathway is required for replication stress tolerance of cancer cells to Chk1 and ATR inhibitors. NPJ Breast Cancer 7:152 (2021). PubMed: 34857765
- Laroche-Clary A et al. ATR Inhibition Broadly Sensitizes Soft-Tissue Sarcoma Cells to Chemotherapy Independent of Alternative Lengthening Telomere (ALT) Status. Sci Rep 10:7488 (2020). PubMed: 32366852