Product nameAnti-Chk1 (phospho S301) antibody
See all Chk1 primary antibodies
DescriptionRabbit polyclonal to Chk1 (phospho S301)
Specificityab61023 detects endogenous levels of Chk1 only when phosphorylated at serine 301.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of serine 301 (D-F-SP-P-V).
- Extracts from 293 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab61023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).|
- Information by UniProt
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
All lanes : Anti-Chk1 (phospho S301) antibody (ab61023) at 1/500 dilution
Lane 1 : Extracts from 293 cells
Lane 2 : Extracts from 293 cells with Immunizing peptide
Predicted band size: 54 kDa
Observed band size: ~100,50 kDa why is the actual band size different from the predicted?
ab61023 has not yet been referenced specifically in any publications.