Key features and details
- Rabbit polyclonal to Chk1 (phospho S317)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Chk1 (phospho S317) antibody
See all Chk1 primary antibodies
DescriptionRabbit polyclonal to Chk1 (phospho S317)
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Synthetic peptide within Human Chk1 (internal sequence) (phospho S317). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: O14757
- WB: HCT 116 cells treated with cisplatin. ICC/IF: HeLa cells treated with UVC light.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 54 kDa.|
|ICC/IF||1/100 - 1/1000.|
- Information by UniProt
- C85740 antibody
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All lanes : Anti-Chk1 (phospho S317) antibody (ab226929) at 1/500 dilution
Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell extract
Lane 2 : HCT 116, treated with 30 uM cisplatin for 24 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 54 kDa
Left panels: Control 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Chk1 (phospho S317) (green) using ab226929 at 1/500 dilution in ICC/IF.
Right panels: UVC-exposed (100 J/m2 followed by 8 hours recovery) 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Chk1 (phospho S317) (green) using ab226929 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226929 has not yet been referenced specifically in any publications.