Product nameAnti-Chk1 (phospho S317) antibody
See all Chk1 primary antibodies
DescriptionRabbit polyclonal to Chk1 (phospho S317)
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Synthetic peptide within Human Chk1 (internal sequence) (phospho S317). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: O14757
- WB: HCT 116 cells treated with cisplatin. ICC/IF: HeLa cells treated with UVC light.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 54 kDa.|
|ICC/IF||1/100 - 1/1000.|
- Information by UniProt
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All lanes : Anti-Chk1 (phospho S317) antibody (ab226929) at 1/500 dilution
Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell extract
Lane 2 : HCT 116, treated with 30 uM cisplatin for 24 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 54 kDa
Left panels: Control 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Chk1 (phospho S317) (green) using ab226929 at 1/500 dilution in ICC/IF.
Right panels: UVC-exposed (100 J/m2 followed by 8 hours recovery) 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Chk1 (phospho S317) (green) using ab226929 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
ab226929 has not yet been referenced specifically in any publications.