Key features and details
- Rabbit polyclonal to Chk1 (phospho S317)
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Chk1 (phospho S317) antibody
See all Chk1 primary antibodies
DescriptionRabbit polyclonal to Chk1 (phospho S317)
SpecificityChk1(Phospho-Ser317) Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 317.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 317.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.88% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab38518 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab38518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution (PMID 19390600).
WB: 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Information by UniProt
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
All lanes : Anti-Chk1 (phospho S317) antibody (ab38518) at 1/500 dilution
Lane 1 : Extracts from MCF7 cells (5-30ug of total protein)
Lane 2 : Extracts from COLO cells (5-30ug of total protein)
All lanes : Alkaline Phosphatase Goat Anti Rabbit IgG (H+L)
Predicted band size: 54 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab38518 has been referenced in 2 publications.
- Jiang D et al. TET3-mediated DNA oxidation promotes ATR-dependent DNA damage response. EMBO Rep 18:781-796 (2017). PubMed: 28325772
- Kadaja M et al. Mechanism of genomic instability in cells infected with the high-risk human papillomaviruses. PLoS Pathog 5:e1000397 (2009). ICC/IF ; Human . PubMed: 19390600