Product nameAnti-Chk1 (phospho S317) antibody
See all Chk1 primary antibodies
DescriptionRabbit polyclonal to Chk1 (phospho S317)
SpecificityChk1(Phospho-Ser317) Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 317.
Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
Species reactivityReacts with: Rat, Human
Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 317.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 150% Sodium chloride, PBS (without Mg2+ and Ca2+), pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab38518 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab38518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution (PMID 19390600).
WB: 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Information by UniProt
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All lanes : Anti-Chk1 (phospho S317) antibody (ab38518) at 1/500 dilution
Lane 1 : Extracts from MCF7 cells (5-30ug of total protein)
Lane 2 : Extracts from COLO cells (5-30ug of total protein)
All lanes : Alkaline Phosphatase Goat Anti Rabbit IgG (H+L)
Predicted band size: 54 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?