Recombinant Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Chk1S317-G1] to Chk1 (phospho S317)
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human, African green monkey
Related conjugates and formulations
Overview
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Product name
Anti-Chk1 (phospho S317) antibody [Chk1S317-G1]
See all Chk1 primary antibodies -
Description
Rabbit monoclonal [Chk1S317-G1] to Chk1 (phospho S317) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey -
Immunogen
Synthetic peptide within Human Chk1 (phospho S317). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O14757 -
Positive control
- WB: COS7 (treated with UV + TPA) cell extract. Flow cyt: K562 and NIH/3T3 cells (both treated with IFNa + IL4 + pervanadate); K562 cells (treated with pervanadate).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
Chk1S317-G1 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab278717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.01 µg/ml. Predicted molecular weight: 54 kDa.
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Flow Cyt |
Use a concentration of 0.001 - 1 µg/ml.
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Notes |
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WB
Use a concentration of 0.01 µg/ml. Predicted molecular weight: 54 kDa. |
Flow Cyt
Use a concentration of 0.001 - 1 µg/ml. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 1111 Human
- Entrez Gene: 12649 Mouse
- Omim: 603078 Human
- SwissProt: O14757 Human
- SwissProt: O35280 Mouse
- Unigene: 24529 Human
- Unigene: 16753 Mouse
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Alternative names
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
see all
Images
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Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or using 0.1 µg/mL of isotype control (orange) or treated with pervanadate and using 0.1 µg/mL of ab278717 (green) or pervanadate and blocked with phospho peptide (black) or pervanadate and blocked with non-phospho peptide (purple).
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All lanes : Anti-Chk1 (phospho S317) antibody [Chk1S317-G1] (ab278717) at 0.01 µg/ml
Lane 1 : COS7 cell extract treated with UV+ TPA
Lane 2 : COS7 cell extract untreated with UV+ TPA
Predicted band size: 54 kDa -
Peptide blocking flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (light blue) or using 0.1 µg/mL of isotype control (orange) or treated with IFNa + IL4 + pervanadate and using 0.1 µg/mL of ab278717 (green) or IFNa + IL4 + Pv and blocked with phospho peptide (black) or IFNa + IL4 + Pv and blocked with non-phospho peptide (purple).
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Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL4 + pervanadate (green) using ab278717 at 0.1 µg/mL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab278717 has been referenced in 1 publication.
- Wang T et al. TOP2A promotes proliferation and metastasis of hepatocellular carcinoma regulated by miR-144-3p. J Cancer 13:589-601 (2022). PubMed: 35069905