Overview

  • Product name

    Anti-Chk1 (phospho S345) antibody
    See all Chk1 primary antibodies
  • Description

    Rabbit polyclonal to Chk1 (phospho S345)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Drosophila melanogaster
  • Immunogen

    The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 345 (S-F-SP-Q-P).

  • Positive control

    • Human breast carcinoma tissue
  • General notes


    Chk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Primary antibody notes

    Chk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200. See Abreview.
IHC-Fr Use at an assay dependent concentration. PubMed: 20978077Fixed in -20°C methanol.
IHC-P 1/50 - 1/100.
ELISA 1/10000.

Target

Images

  • ab47318 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
  • ab47318 (1/200) detecting Chk1 in HeLa cells in conjunction with a goat anti-rabbit secondary antibody conjugated to Cy3® (red). Cells were also counterstained with DAPI in order to highlight the nucleus (blue), treated with Bleomycin and incubated with an antibody against Histone H2AX in order to create and expose DNA double strand breaks (green). Please refer to abreviews for further details.

    See Abreview

References

This product has been referenced in:

  • Nie ZW  et al. Thiamethoxam inhibits blastocyst expansion and hatching via reactive-oxygen species-induced G2 checkpoint activation in pigs. Cell Signal 53:294-303 (2019). Read more (PubMed: 30145217) »
  • Nasri S  et al. Early Hereditary Diffuse Gastric Cancer (eHDGC) is Characterized by Subtle Genomic Instability and Active DNA Damage Response. Pathol Oncol Res 25:711-721 (2019). Read more (PubMed: 30547291) »
See all 18 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thanks for your email.
Please feel free to call me between hours of 8:30am and 5pm EST. Our Scientific Support is available from 8am - 8pm EST and you can feel free to speak to any of my colleagues about these issues as well.
I look forward to hearing from you.

Read More

Answer

Thank you for completing our recent survey. As you have noted technical difficulties with two antibodies from Abcam, I just wanted to follow up to see how I might help to rectify any issues.
To help me assist you most efficiently, it would be helpful if you could provide a bit more detail regarding the samples, sample preparation methods and experimental protocol used in the WB and IHC experiments. I'm happy to offer any troubleshooting tips that may improve your results. If I can't help to improve your results to meet expectations, I am happy to offer a replacement, credit or refund for these 2 antibodies.
Thanks in advance for the additional information!

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (MRC5VA Lung Epithelial Fibroblasts)
Loading amount
15 µg
Specification
MRC5VA Lung Epithelial Fibroblasts
Gel Running Conditions
Reduced Denaturing (9%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Mr. Darren Arbon

Verified customer

Submitted Apr 14 2011

Answer

Thank you for your enquiry. All the information that we have about the protocol used by K. McManus is already stated on the review. At Abcam, we always give our customers all the information that we have at hand and I am afraid that there is no other information that we can possibly add. Unfortunately, K. McManus has requested not to be contacted by other customers regarding this review, otherwise you would be able to personally ask him for the protocols he used. I have to respect his decision and therefore not disclose his personal contact information. I am sorry I can't be more helpful in this occasion but should you require any other information, please do not hesitate to contact me.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Apr 19 2007

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