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reactivity is an issue: Ab47318 (anti chk1) says effective against mouse, but at least not working in mouse intestinal tissue.for IHC. Ab88661 (anti ANKRD1) NOT work with western on human cell lysates. And the costs of these antibodies are not cheap.
Asked on Jun 11 2012
Thank you for completing our recent survey. As you have noted technical difficulties with two antibodies from Abcam, I just wanted to follow up to see how I might help to rectify any issues.
To help me assist you most efficiently, it would be helpful if you could provide a bit more detail regarding the samples, sample preparation methods and experimental protocol used in the WB and IHC experiments. I'm happy to offer any troubleshooting tips that may improve your results. If I can't help to improve your results to meet expectations, I am happy to offer a replacement, credit or refund for these 2 antibodies.
Thanks in advance for the additional information!
Answered on Jun 11 2012