Product nameAnti-Chk1 (phospho S345) antibody
See all Chk1 primary antibodies
DescriptionRabbit polyclonal to Chk1 (phospho S345)
SpecificitySpecific for Chk1 (phospho S345) in ELISA, with <1% reactivity with the non-modified form of the immunogen.
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
- This antibody gave a positive signal in Hydroxyurea Treated (48hr, 2uM) Hela whole cell lysate. IHC-P: FFPE Human cervix normal.
Please note we cannot guarantee ICC/IF, however should you use this application for this product then please use a specific protocol which can be found in the protocols section of our datasheet here.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab58567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).|
|IHC-P||Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
- Information by UniProt
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
All lanes : Anti-Chk1 (phospho S345) antibody (ab58567) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate - Untreated
Lane 2 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Additional bands at: 105 kDa, 42 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab58567 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
IHC image of ab58567 staining Human Cervical Carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58567, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
This product has been referenced in:
- Bhattacharyya R et al. Coralyne, a protoberberine alkaloid, causes robust photosenstization of cancer cells through ATR-p38 MAPK-BAX and JAK2-STAT1-BAX pathways. Chem Biol Interact 285:27-39 (2018). Read more (PubMed: 29486184) »
- Al-Subhi N et al. Targeting ataxia telangiectasia-mutated- and Rad3-related kinase (ATR) in PTEN-deficient breast cancers for personalized therapy. Breast Cancer Res Treat 169:277-286 (2018). Read more (PubMed: 29396668) »