Recombinant Anti-Chk2 antibody [EPR19236] (ab199031)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19236] to Chk2
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Chk2 antibody [EPR19236]
See all Chk2 primary antibodies -
Description
Rabbit monoclonal [EPR19236] to Chk2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse spleen and colon lysates; NIH/3T3, C2C12 and bEND.3 whole cell lysates. IHC: Mouse spleen and testis tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19236 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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IP |
1/40.
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Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
IP
1/40. |
Target
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Function
Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. -
Tissue specificity
High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues. -
Involvement in disease
Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500]. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
Contains 1 FHA domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated by PLK4. -
Cellular localization
Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53. - Information by UniProt
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Database links
- Entrez Gene: 50883 Mouse
- SwissProt: Q9Z265 Mouse
- Unigene: 279308 Mouse
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Alternative names
- CDS 1 antibody
- Cds1 antibody
- Cds1 homolog antibody
see all
Images
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All lanes : Anti-Chk2 antibody [EPR19236] (ab199031) at 1/1000 dilution
Lane 1 : C2C12 (mouse myoblast cell line) whole cell lysate
Lane 2 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 15 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
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Chk2 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate with ab199031 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199031 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Exposure time 3 minutes.
Lane 1: NIH/3T3 whole cell lysate 10 µg (Input).Lane 2: ab199031 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199031 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes : Anti-Chk2 antibody [EPR19236] (ab199031) at 1/1000 dilution
All lanes :
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Observed band size: 61 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Chk2 with ab199031 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101, ready to use). Nuclear staining on spermatogonia of mouse testis is observed (PMID: 12192050, 11593395). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101, ready to use.
Nuclear staining on spermatogonia of mouse testis (PMID: 12192050, PMID: 11593395).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Chk2 with ab199031 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101, ready to use). Nuclear staining on mouse spleen is observed (PMID: 12192050, 11593395). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101, ready to use.
Nuclear staining on mouse spleen (PMID: 12192050, PMID: 11593395).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Chk2 antibody [EPR19236] (ab199031) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse colon tissue lysate
Lane 3 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab199031 has not yet been referenced specifically in any publications.