Overview

  • Product name

    Anti-Chk2 antibody [EPR19236]
    See all Chk2 primary antibodies
  • Description

    Rabbit monoclonal [EPR19236] to Chk2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse Chk2 aa 1-250. The exact sequence is proprietary.
    Database link: Q9Z265

  • Positive control

    • WB: Mouse spleen and colon lysates; NIH/3T3, C2C12 and bEND.3 whole cell lysates. IHC: Mouse spleen and testis tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IP 1/40.

Target

  • Function

    Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
  • Tissue specificity

    High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
  • Involvement in disease

    Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
    Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated by PLK4.
  • Cellular localization

    Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDS 1 antibody
    • Cds1 antibody
    • Cds1 homolog antibody
    • Checkpoint kinase 2 antibody
    • Checkpoint like protein CHK2 antibody
    • CHEK 2 antibody
    • Chek2 antibody
    • Chk 2 antibody
    • CHK2 checkpoint homolog (S. pombe) antibody
    • CHK2 checkpoint homolog antibody
    • CHK2_HUMAN antibody
    • hCds1 antibody
    • HuCds 1 antibody
    • LFS 2 antibody
    • LFS2 antibody
    • PP1425 antibody
    • RAD 53 antibody
    • RAD53 antibody
    • Rad53 homolog antibody
    • Serine/threonine protein kinase Chk2 antibody
    • Serine/threonine-protein kinase Chk2 antibody
    see all

Images

  • All lanes : Anti-Chk2 antibody [EPR19236] (ab199031) at 1/1000 dilution

    Lane 1 : C2C12 (mouse myoblast cell line) whole cell lysate
    Lane 2 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa


    Exposure time: 15 seconds


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Chk2 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate with ab199031 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199031 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Exposure time 3 minutes.


    Lane 1: NIH/3T3 whole cell lysate 10 µg (Input).

    Lane 2: ab199031 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199031 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.



    All lanes : Anti-Chk2 antibody [EPR19236] (ab199031) at 1/1000 dilution

    All lanes :

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Observed band size: 61 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Chk2 with ab199031 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101, ready to use). Nuclear staining on spermatogonia of mouse testis is observed (PMID: 12192050, 11593395). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101, ready to use.

    Nuclear staining on spermatogonia of mouse testis (PMID: 12192050, PMID: 11593395).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Chk2 with ab199031 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101, ready to use). Nuclear staining on mouse spleen is observed (PMID: 12192050, 11593395). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101, ready to use.

    Nuclear staining on mouse spleen (PMID: 12192050, PMID: 11593395).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • All lanes : Anti-Chk2 antibody [EPR19236] (ab199031) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Mouse colon tissue lysate
    Lane 3 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab199031 has not yet been referenced specifically in any publications.

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