Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Chk2 antibody [EPR5528] (ab133505)

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Overview

  • Product name

    Anti-Chk2 antibody [EPR5528]
    See all Chk2 primary antibodies

  • Description

    Rabbit monoclonal [EPR5528] to Chk2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Chk2 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • SH-SY5Y, HeLa, HT29 and 293T cell lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

    • Tissue specificity

      High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

    • Involvement in disease

      Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
      Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
      Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].

    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
      Contains 1 FHA domain.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Phosphorylated by PLK4.

    • Cellular localization

      Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
    • Information by UniProt

    • Database links

      • Alternative names

        • CDS 1 antibody
        • Cds1 antibody
        • Cds1 homolog antibody
        • Checkpoint kinase 2 antibody
        • Checkpoint like protein CHK2 antibody
        • CHEK 2 antibody
        • Chek2 antibody
        • Chk 2 antibody
        • CHK2 checkpoint homolog (S. pombe) antibody
        • CHK2 checkpoint homolog antibody
        • CHK2_HUMAN antibody
        • hCds1 antibody
        • HuCds 1 antibody
        • LFS 2 antibody
        • LFS2 antibody
        • PP1425 antibody
        • RAD 53 antibody
        • RAD53 antibody
        • Rad53 homolog antibody
        • Serine/threonine protein kinase Chk2 antibody
        • Serine/threonine-protein kinase Chk2 antibody
        see all

      Images

      • Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)
        Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)

        Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
        Lanes 2, 6 and 10: Chk2 knockout HAP1 cell lysate (20 µg)
        Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
        Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)
        Lanes 1, 2, 3 and 4: Green signal from target - ab133505 observed at 62 kDa
        Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
        Lanes 9, 10, 11 and 12: Merged (red and green) signal
        ab133505 was shown to specifically react with Chk2 when Chk2 knockout samples were used. Wild-type and Chk2 knockout samples were subjected to SDS-PAGE. ab133505 and ab8245 (loading control to GAPDH) were diluted 1/10000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

      • Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)
        Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)
        All lanes : Anti-Chk2 antibody [EPR5528] (ab133505) at 1/10000 dilution

        Lane 1 : SH-SY5Y lysate
        Lane 2 : HeLa lysate
        Lane 3 : HT29 lysate
        Lane 4 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 61 kDa
        Observed band size: 61 kDa

      • Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)
        Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)

        Lanes 1: Wild-type HAP1 cell lysate (20 µg)
        Lanes 2: Chk2 knockout HAP1 cell lysate (20 µg)
        Lanes 3: HeLa cell lysate (20 µg)
        Lanes 4: HEK293 cell lysate (20 µg)

        Lanes 1 - 4: Merged signal (red and green). Green - ab133505 observed at 64 kDa. Red - loading control, ab8245, observed at 37 kDa.

        This western blot image is a comparison between ab133505 and a competitor's rabbit polyclonal antibody.

      • Other - Anti-Chk2 antibody [EPR5528] (ab133505)
        Other - Anti-Chk2 antibody [EPR5528] (ab133505)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      This product has been referenced in:

      • Wrobel L  et al. The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly. Sci Rep 6:27484 (2016). Read more (PubMed: 27265872) »
      See 1 Publication for this product

      Publishing research using ab133505? Please let us know so that we can cite the reference in this datasheet.

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