- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-Chk2 (phospho T68) antibody
See all Chk2 primary antibodies -
DescriptionRabbit polyclonal to Chk2 (phospho T68)
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Host speciesRabbit
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Specificityab3501 was tested using MCF7 cells treated with different amounts of doxorubicin (a DNA damaging agent). As the treatment increased the WB signal at 60kDa representing Chk2 increased, indicating that ab3501 was detecting the phosphorylated form of the protein. It has also been shown by peptide ELISA to be specific for the phosphorylated form of the immunizing peptide versus the non-phosphorylated form.
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee
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Immunogen
Synthetic peptide: (C)ETVSTpQELYS, corresponding to amino acids 64-73 of Chk2.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab3501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/200 - 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
Target
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FunctionRegulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
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Tissue specificityHigh expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
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Involvement in diseaseDefects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500]. -
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
Contains 1 FHA domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated by PLK4. -
Cellular localizationNucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
- Information by UniProt
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Database links
- Entrez Gene: 11200 Human
- Entrez Gene: 50883 Mouse
- Entrez Gene: 114212 Rat
- Omim: 604373 Human
- SwissProt: O96017 Human
- SwissProt: Q9Z265 Mouse
- Unigene: 291363 Human
- Unigene: 505297 Human
see all -
Alternative names
- CDS 1 antibody
- Cds1 antibody
- Cds1 homolog antibody
see all
Images
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Western blot - Anti-Chk2 (phospho T68) antibody (ab3501)The Western blot shows lysates from MCF-7 cells treated for 24 hours with increasing concentrations of the DNA damaging agent Doxorubicin (0-10µM). The blot clearly shows that Chk2 phosphorylation is increased with the higher concentrations of Doxorubicin.
Protocols
References
This product has been referenced in:
- Kim SH et al. Mael is essential for cancer cell survival and tumorigenesis through protection of genetic integrity. Oncotarget 8:5026-5037 (2017). WB ; Human . Read more (PubMed: 27926513) »
- Sidler C et al. A role for SUV39H1-mediated H3K9 trimethylation in the control of genome stability and senescence in WI38 human diploid lung fibroblasts. Aging (Albany NY) 6:545-63 (2014). WB . Read more (PubMed: 25063769) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"