Anti-Chk2 (phospho T68) antibody (ab3501)

Reviews (2)Q&A (2)References (8)
Western blot - Anti-Chk2 (phospho T68) antibody (ab3501)

    Key features and details

    • Rabbit polyclonal to Chk2 (phospho T68)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-Chk2 (phospho T68) antibody
      See all Chk2 primary antibodies

    • Description

      Rabbit polyclonal to Chk2 (phospho T68)

    • Host species

      Rabbit

    • Specificity

      ab3501 was tested using MCF7 cells treated with different amounts of doxorubicin (a DNA damaging agent). As the treatment increased the WB signal at 60kDa representing Chk2 increased, indicating that ab3501 was detecting the phosphorylated form of the protein. It has also been shown by peptide ELISA to be specific for the phosphorylated form of the immunizing peptide versus the non-phosphorylated form.

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide: (C)ETVSTpQELYS, corresponding to amino acids 64-73 of Chk2.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3501 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rat
    Chimpanzee
    Application Abreviews Notes
    WB (2)
    1/200 - 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
    Notes
    WB
    1/200 - 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

    Target

    • Function

      Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

    • Tissue specificity

      High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

    • Involvement in disease

      Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
      Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
      Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].

    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
      Contains 1 FHA domain.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Phosphorylated by PLK4.

    • Cellular localization

      Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CDS 1 antibody
      • Cds1 antibody
      • Cds1 homolog antibody
      • Checkpoint kinase 2 antibody
      • Checkpoint like protein CHK2 antibody
      • CHEK 2 antibody
      • Chek2 antibody
      • Chk 2 antibody
      • CHK2 checkpoint homolog (S. pombe) antibody
      • CHK2 checkpoint homolog antibody
      • CHK2_HUMAN antibody
      • hCds1 antibody
      • HuCds 1 antibody
      • LFS 2 antibody
      • LFS2 antibody
      • PP1425 antibody
      • RAD 53 antibody
      • RAD53 antibody
      • Rad53 homolog antibody
      • Serine/threonine protein kinase Chk2 antibody
      • Serine/threonine-protein kinase Chk2 antibody
      see all

    Images

    • Western blot - Anti-Chk2 (phospho T68) antibody (ab3501)
      Western blot - Anti-Chk2 (phospho T68) antibody (ab3501)

      The Western blot shows lysates from MCF-7 cells treated for 24 hours with increasing concentrations of the DNA damaging agent Doxorubicin (0-10µM). The blot clearly shows that Chk2 phosphorylation is increased with the higher concentrations of Doxorubicin.

    References (8)

    Publishing research using ab3501? Please let us know so that we can cite the reference in this datasheet.

    ab3501 has been referenced in 8 publications.

    • Wang J  et al. Bacterial quorum-sensing signal IQS induces host cell apoptosis by targeting POT1-p53 signalling pathway. Cell Microbiol 21:e13076 (2019). PubMed: 31254473
    • Pan Z  et al. Cinobufagin Induces Cell Cycle Arrest at the G2/M Phase and Promotes Apoptosis in Malignant Melanoma Cells. Front Oncol 9:853 (2019). PubMed: 31552178
    • Wang D  et al. Colonic Lysine Homocysteinylation Induced by High-Fat Diet Suppresses DNA Damage Repair. Cell Rep 25:398-412.e6 (2018). PubMed: 30304680
    • Kim SH  et al. Mael is essential for cancer cell survival and tumorigenesis through protection of genetic integrity. Oncotarget 8:5026-5037 (2017). WB ; Human . PubMed: 27926513
    • Higashiguchi M  et al. Clarifying the biological significance of the CHK2 K373E somatic mutation discovered in The Cancer Genome Atlas database. FEBS Lett 590:4275-4286 (2016). PubMed: 27716909
    • Sidler C  et al. A role for SUV39H1-mediated H3K9 trimethylation in the control of genome stability and senescence in WI38 human diploid lung fibroblasts. Aging (Albany NY) 6:545-63 (2014). WB . PubMed: 25063769
    • Bertout JA  et al. HIF2{alpha} inhibition promotes p53 pathway activity, tumor cell death, and radiation responses. Proc Natl Acad Sci U S A 106:14391-6 (2009). WB ; Human . PubMed: 19706526
    • Chabalier-Taste C  et al. BRCA1 is regulated by Chk2 in response to spindle damage. Biochim Biophys Acta 1783:2223-33 (2008). PubMed: 18804494

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Chk2 (phospho T68) antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse prostate cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    20 µg
    Treatment
    10 Gy irradiation
    Specification
    mouse prostate cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 11 2021

    Western blot abreview for Anti-Chk2 (phospho T68) antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Mouse Cell lysate - whole cell (Neural stem cells)
    Specification
    Neural stem cells
    Treatment
    MMS 0,02% Adriamicine 0,5 ´g /mL
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Dr. J Igual

    Verified customer

    Submitted May 08 2014

    Question


    I have been looking for a phospho-Chk2 (Thr68) antibody that works on material from rats or mice, for use in WB or IHC. I see on your website that you have 3 antibodies , no.s’ ab38461, ab85743 and ab3501 in your catalogue, and I just wondered if you could tell me whether they would work in rodent tissue? I know the latter 2 are ‘predicted’ to work in rat and mouse, but I wondered if anyone had commented saying that this has been confirmed?

    If this remains unknown, would it be possible to one of the antibodies at a discounted rate, and I could test it for you, to see if it does work?

    Many thanks

    Read More
    Answer

    Thank you very much for your interest in our antibodies against Chk2 phospho Thr68.
    To our knowledge, all these three antibodies have not been tested in rodent species. All these three antibodies are however predicted to react against the mouse and the rat protein, as the sequence around the Thr68 is quite conserved. Please see here the alignment:
    http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120420-160419-0159-55604608-oy
    The crossreaction needs however to be tested experimentally and I can make you an interesting offer if you would like to test this: I can offer a discount off a future purchase if you buy one of these antibodies now, test it in rodent species and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.
    If you would like to test one of these antibodies, I would recommend to test the ab38461, as it is the only of these three antibodies which has been tested in Western blot and IHC-P. I leave this decision however up to you, and I am happy to provide the code for testing also another one of these three antibodies on rat and mouse.
    If you are interested in this offer, please follow these steps:
    1. Reply to this e-mail to let me know which antibody you would like to test in rat and mouse. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.
    2. Purchase the antibody either by phone, fax, or online (www.abcam.com).
    3. Test it in mouse and rat.
    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the anti Chk2 phospho Thr68 turns out to be unsuitable for rat or mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.
    Please let me know if you have any questions about this offer and I would be happy to help you further.
    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Question

    I am looking for a phosphorylated checkpoint kinase 2 antibody for mouse at threonine 68. On the data sheet of ab3501 it stated it was specific for humans and based on 90% homology may react with mouse. I was wondering if you had any feedback that this antibody does react with mouse samples.

    Read More
    Answer

    Thank you for your enquiry. We have not received any feedback from customers who have tried this antibody for cross-reactivity with mouse. Although it is predicted to cross-react due to 90% sequence homology, we cannot guarantee it. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 points will be offered for an image). Please let me know if you require any further assistance.

    Read More

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