Overview

  • Product name

    Anti-Chk2 (phospho T68) antibody [Y171] - BSA and Azide free
    See all Chk2 primary antibodies
  • Description

    Rabbit monoclonal [Y171] to Chk2 (phospho T68) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, Dot blot, WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Chk2. The exact sequence is proprietary.

  • Positive control

    • Cell lysates from HEK-293 cells treated with UV and/or phosphatase.
  • General notes

    ab239808 is the carrier-free version of ab32148 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239808 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 62 kDa (predicted molecular weight: 61 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC.
  • Target

    • Function

      Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
    • Tissue specificity

      High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
    • Involvement in disease

      Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
      Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
      Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
      Contains 1 FHA domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylated by PLK4.
    • Cellular localization

      Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
    • Information by UniProt
    • Database links

    • Alternative names

      • CDS 1 antibody
      • Cds1 antibody
      • Cds1 homolog antibody
      • Checkpoint kinase 2 antibody
      • Checkpoint like protein CHK2 antibody
      • CHEK 2 antibody
      • Chek2 antibody
      • Chk 2 antibody
      • CHK2 checkpoint homolog (S. pombe) antibody
      • CHK2 checkpoint homolog antibody
      • CHK2_HUMAN antibody
      • hCds1 antibody
      • HuCds 1 antibody
      • LFS 2 antibody
      • LFS2 antibody
      • PP1425 antibody
      • RAD 53 antibody
      • RAD53 antibody
      • Rad53 homolog antibody
      • Serine/threonine protein kinase Chk2 antibody
      • Serine/threonine-protein kinase Chk2 antibody
      see all

    Images

    • ab32148 (purified) at 1:30 dilution (2µg) immunoprecipitating Chk2 in HEK-293 treated with UV whole cell lysate.
      Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) treated with UV whole cell lysate 10µg
      Lane 2 (+): ab32148 & HEK-293 treated with UV whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32148 in HEK-293 treated with UV whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32148).

    • ab32148 at 1/20 dilution immunoprecipitating Chk2 (phospho T68) in HEK-293 (human embryonic kidney) whole cell lysate observed at 70kDa (lanes 1 and 2).

      Lane 1 (input): HEK-293 cells treated with UV at 60J/m2 for 2 hours. Whole cell lysate 10μg

      Lane 2 (+): ab32148 + HEK-293 treated with UV at 60J/m2 for 2 hours. Whole cell lysate

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32148 in HEK- treated with UV at 60J/m2 for 2 hours. Whole cell lysate

      For western blotting, ab32148 at 1/500 dilution was used followed by VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32148).

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32148).

    • Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti-Phospho-Chkr2 (pT68) RabMAb (ab32148) dilution 1:500

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32148).

      This image was generated using the unpurified version of the product. 

    References

    ab239808 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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