Key features and details
- Goat polyclonal to Chlamydia trachomatis MOMP
- Suitable for: FM
- Isotype: IgG
Product nameAnti-Chlamydia trachomatis MOMP antibody
See all Chlamydia trachomatis MOMP primary antibodies
DescriptionGoat polyclonal to Chlamydia trachomatis MOMP
Major Outer Membrane Protein (MOMP). Reacts with all serovars (A-K, L1-L3). Does not react with C. psittacii or C. pneumoniae in MIF. Negative against HEp-2 cells and egg yolk sac.
Tested applicationsSuitable for: FMmore details
Full length native protein (purified) corresponding to Chlamydia trachomatis MOMP. Strain L2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityIon Exchange Chromatography
Purification notes> 95% pure. Sodium sulfate precipitation and ion-exchange chromatography.
Our Abpromise guarantee covers the use of ab252752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.
Suitable for use in fluorescence microscopy. Also suitable for conjugation purposes. Each laboratory should determine an optimum working titer for use in its particular application.
RelevanceChlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cell's nucleus. Chlamydia trachomatis is an intracellular organism that has a genome size of approximately 500-1000 kilobases and contains both RNA and DNA. The organism is also extremely temperature sensitive and must be refrigerated at 4°C as soon as a sample is obtained. Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T cells, and B cells. It also exists as 15 different serotypes. These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3). Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be Gram negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it. It may, however, contain a carboxylated sugar other than muramic acid. The proposed structure consists of a major outer membrane protein cross linked with disulfide bonds. It also contains cysteine rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival (Hatch 1996). Chlamydia usually infects the cervix and fallopian tubes of women and the urethra of men. Chlamydial infections are believed to be one of the most common of all STDs. It is generally thought that in a population of 15 million, there are up to 300,000 cases of chlamydia each year. Thus, there are many undiagnosed cases of chlamydia in the community. It has been estimated that the true prevalence of chlamydia in the sexually active population may be in the order of 5% to 10%. Chlamydia is one of the leading causes of blindness in underdeveloped countries.
Cellular localizationOuter membrane; multi pass membrane protein.
- Major Outer Membrane Protein antibody
- MOMP antibody
- omp1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab252752 has not yet been referenced specifically in any publications.