Anti-Chloramphenicol antibody (ab35658)
Key features and details
- Sheep polyclonal to Chloramphenicol
- Suitable for: ELISA, FPIA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Chloramphenicol antibody
See all Chloramphenicol primary antibodies -
Description
Sheep polyclonal to Chloramphenicol -
Host species
Sheep -
Tested applications
Suitable for: ELISA, FPIAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Aromatic amino derivative of chloramphenicol (prepared by reduction of the nitro group by high-pressure hydrogenation) conjugated to KLH using the diazotisation procedure.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Prepared by Ammonium Sulphate fractionation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab35658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | ||
FPIA |
Notes |
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ab35658 was used in a simple indirect ELISA with 400 ng of Chloramphenicol-BSA per well. The titres detailed above were elucidated using an HRP labelled secondary antibody/TMB detection system.
FPIA: 1/1500.
When tested by polarisation fluoroimmunoassay, the final titre dilution was found to be 0.5 ml tracer + 0.5 ml diluted antibody. Tracer is aromatic amino derivative of chloramphenicol labelled by reaction with fluorescein isothiocyanate (10 nmol/L).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Chloramphenicol is an antibiotic that was derived from the bacterium Streptomyces venezuelae. It was the first antibiotic to be manufactured synthetically on a large scale. Chloramphenicol is effective against a wide variety of microorganisms, but due to serious side effects (eg damage to the bone marrow) in humans, it is usually reserved for the treatment of serious and life threatening infections (eg typhoid fever). It is also used in eye drops or ointment to treat bacterial conjunctivitis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab35658 has not yet been referenced specifically in any publications.