Key features and details
- Sheep polyclonal to Chloramphenicol
- Suitable for: ELISA, FPIA
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Chloramphenicol antibody
See all Chloramphenicol primary antibodies
DescriptionSheep polyclonal to Chloramphenicol
Tested applicationsSuitable for: ELISA, FPIAmore details
Species reactivityReacts with: Species independent
Aromatic amino derivative of chloramphenicol (prepared by reduction of the nitro group by high-pressure hydrogenation) conjugated to KLH using the diazotisation procedure.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPrepared by Ammonium Sulphate fractionation.
Our Abpromise guarantee covers the use of ab35658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab35658 was used in a simple indirect ELISA with 400 ng of Chloramphenicol-BSA per well. The titres detailed above were elucidated using an HRP labelled secondary antibody/TMB detection system.
When tested by polarisation fluoroimmunoassay, the final titre dilution was found to be 0.5 ml tracer + 0.5 ml diluted antibody. Tracer is aromatic amino derivative of chloramphenicol labelled by reaction with fluorescein isothiocyanate (10 nmol/L).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceChloramphenicol is an antibiotic that was derived from the bacterium Streptomyces venezuelae. It was the first antibiotic to be manufactured synthetically on a large scale. Chloramphenicol is effective against a wide variety of microorganisms, but due to serious side effects (eg damage to the bone marrow) in humans, it is usually reserved for the treatment of serious and life threatening infections (eg typhoid fever). It is also used in eye drops or ointment to treat bacterial conjunctivitis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab35658 has not yet been referenced specifically in any publications.