Overview

  • Product name

    Chloride Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Quantitative
  • Sensitivity

    > 0.4 mM
  • Range

    20 nmol/well - 120 nmol/well
  • Assay time

    0h 20m
  • Product overview

    Abcam's Chloride Assay Kit provides a quick, simple method for quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with water. The assay is based upon the competition of Hg2+ and Fe2+ for TPTZ. The preferred Hg-TPTZ adduct exhibits no color. In the presence of Chloride, Hg2+ forms HgCl2 freeing up TPTZ which then binds the available Fe2+ giving a very intense absorbance with a λmax~ 620nm. The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Chloride is the anionic form of chlorine. It is the most common of the anions found in living organisms. Chloride ions play a variety of important physiological roles. Chloride channels are found in a variety of cells and are responsible for setting resting cell membrane potential and regulating cell volume. In the nervous system, the action of glycine and GABA are related to chloride levels in specific neurons. Chloride is also instrumental in maintaining the acid-base balance in blood. The kidneys are instrumental in closely regulating serum chloride levels. There are a number of pathologies associated with defective chloride transport; the most well-known being Cystic Fibrosis, caused by a mutation in CFTR a membrane chloride transporter.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at room temperature. Please refer to protocols.
  • Components Identifier 100 tests
    Chloride Reagent WM 1 x 15ml
    Chloride Standard (10 µmol) (Lyophilized) Yellow 1 vial
  • Research areas

  • Relevance

    Chloride is the anionic form of chlorine. It is the most common of the anions found in living organisms. Chloride ions play a variety of important physiological roles. Chloride channels are found in a variety of cells and are responsible for setting resting cell membrane potential and regulating cell volume. In the nervous system, the action of glycine and GABA are related to chloride levels in specific neurons. Chloride is also instrumental in maintaining the acid-base balance in blood. The kidneys are instrumental in closely regulating serum chloride levels. There are a number of pathologies associated with defective chloride transport; the most well-known being Cystic Fibrosis, caused by a mutation in CFTR a membrane chloride transporter.

Images

  • Chloride measured in biological fluids. Samples were diluted 3-27 fold.

  • Chloride Standard Curve: Assays were performed following the kit protocol.

Protocols

References

ab83372 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

The lab let me know the following:

The chloride reagent is bluish in colour to begin with. The final colour developed is going to be intense blue which can be detected at 620 nm.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you again for your call today.

I have just received an update from the lab and wanted to forward this information on to you. The reagent should be blue, but it will turn a darker shade of blue during the reaction, not red as I previously wrote. The absorbance will be in the red spectrum, at 620 nm.

I hope this will be helpful, but please let me know if you have further questions.

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Question
Answer

Thank you for your call today and for your question.

I've been in touch with the scientists in the lab, and the reagent should be blue. The reaction end-product will be red.

I hope this information will be useful, but please let me know if you have further questions or if there is anything else that we can do for you, and I'll be happy to help.

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