Anti-Chloride Channel 5 antibody [N237/24] (ab174937)

Overview

  • Product name
    Anti-Chloride Channel 5 antibody [N237/24]
    See all Chloride Channel 5 primary antibodies
  • Description
    Mouse monoclonal [N237/24] to Chloride Channel 5
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Positive control
    • IHC-P: FFPE human kidney tissue sections.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab174937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function.
  • Tissue specificity
    Kidney. Moderately expressed in aortic vascular smooth muscle and endothelial cells, and at a slightly higher level in the coronary vascular smooth muscle.
  • Involvement in disease
    Hypophosphatemic rickets, X-linked recessive
    Nephrolithiasis 2
    Nephrolithiasis 1
    Low molecular weight proteinuria with hypercalciuria and nephrocalcinosis
  • Sequence similarities
    Belongs to the chloride channel (TC 2.A.49) family. ClC-5/CLCN5 subfamily.
    Contains 2 CBS domains.
  • Post-translational
    modifications
    Ubiquitinated by NEDD4L in the presence of albumin; which promotes endocytosis and proteasomal degradation.
  • Cellular localization
    Golgi apparatus membrane. Endosome membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride channel protein 5 antibody
    • Chloride channel voltage sensitive 5 antibody
    • Chloride transporter ClC-5 antibody
    • ClC-5 antibody
    • CLC5 antibody
    • CLCK2 antibody
    • CLCN5 antibody
    • CLCN5_HUMAN antibody
    • DENTS antibody
    • H(+)/Cl(-) exchange transporter 5 antibody
    • hCIC-K2 antibody
    • NPHL1 antibody
    • NPHL2 antibody
    • Voltage gated chloride ion channel CLCN5 antibody
    • XLRH antibody
    • XRN antibody
    see all

Images

  • IHC image of Chloride Channel 5 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab174937, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab174937 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab174937.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up