Overview

  • Product name
    Chloride Channel Assay Kit (Colorimetric)
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Product overview

    Abcam’s Chloride Channel Assay Kit (Colorimetric) (ab176767) provides a sensitive and robust colorimetric method for studying chloride channels. The assay is based on our proprietary iodide indicator (Iodide Sensor Blue dye) to measure iodide concentration, as low as 30 nM of iodide can be detected. Iodide Sensor Blue dye forms a blue complex with iodide, which has absorption spanning from the UV to 700 nm. Thus a few absorption wavelengths can be used for monitoring the iodide-dependent color change.

    Abcam's Chloride Channel Assay Kit (Colorimetric) (ab176767) provides an optimized assay method for monitoring chloride channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. 

  • Notes

    Chloride channels have a variety of important physiological and cellular functions that include regulation of pH, volume homeostasis, organic solute transport, cell migration, cell proliferation and differentiation. Chloride channels represent valuable drug targets. A number of chronic disease states such as cystic fibrosis and Bartter's syndrome are due to defects in chloride channel functions. However, the existing technologies for screening chloride channel modulators are a compromise between throughput, sensitivity and physiological relevance.

Properties

Images

  • Sodium iodide (Nal) dose response was measured with Abcam's Chloride Channel Assay Kit (Colorimetric) (ab176767) in a 96-well black wall/clear bottom plate. As low as 30 nM of Nal was detected with 10 minutes incubation time (n = 3).

Protocols

References

ab176767 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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