Overview

  • Product name

    Chloroplast Isolation Kit
  • Product overview

    Chloroplast Isolation Kit (ab234623) provides an easy method to isolate chloroplasts from a broad range of plant types including dicots, monocots, and conifers. The isolated chloroplasts can be used to study photosynthetic processes and as starting materials for chloroplast membrane, protein, chloroplast DNA and chloroplast RNA isolations.


     

  • Notes

    Chloroplasts are membrane-bound organelles found in plant and algal cells which perform the function of photosynthesis. Plant chloroplasts are lens-shaped, usually 5-10 µm in diameter and 1-3 µm thick. Although leaves contain the majority of plants’ chloroplasts, they can also be found in stems, seeds, and un-ripened fruit.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 g
    BSA (10% solution) 1 x 10ml
    Chloroplast Isolation Buffer, 2X 1 x 500ml
    Density Gradient Medium 1 x 50ml
    DTT (1 M) 1 x 1ml

Images

  • (a) Debris pellet from first centrifugation at step 7.1.6; (b) Supernatant from step 7.1.7; (c) Intact chloroplasts from step 7.2.3; (d) Broken chloroplasts from step 7.2.3.

  • (a) Spider plant (Chlorophytum comosim); (b) Corn husks (Zea mays); (c) Pine needles (Pinus muricata); (d) Cypress needles (Cupressus sempervirens); (e) Juniper (Juniperus species); (f) Lettuce (Lactuca sativa); (g) Stapelia variegata; (h) Ficus species.

Protocols

References

ab234623 has not yet been referenced specifically in any publications.

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