Overview

  • Product name

    Anti-CHM antibody [EPR7142]
    See all CHM primary antibodies
  • Description

    Rabbit monoclonal [EPR7142] to CHM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human CHM (UniProt ID: P24386).

  • Positive control

    • HeLa, Y79, A431, and 293T cell lysates. A431 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 73 kDa).
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Binds unprenylated Rab proteins, presents it to the catalytic Rab GGTase dimer, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Also a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation.
    • Involvement in disease

      Defects in CHM are the cause of choroideremia (CHM) [MIM:303100]. An X-linked recessive disease characterized by a slowly progressive degeneration of the choroid, photoreceptors, and retinal pigment epithelium. Affected males develop night blindness in their teenage years followed by loss of peripheral vision and complete blindness at middle age. Carrier females are generally asymptomatic but funduscopic examination often shows patchy areas of chorioretinal atrophy.
    • Sequence similarities

      Belongs to the Rab GDI family.
    • Information by UniProt
    • Database links

    • Alternative names

      • CHM antibody
      • Chm antibody
      • Choroideraemia protein antibody
      • Choroideremia antibody
      • DXS540 antibody
      • FLJ38564 antibody
      • GGTA antibody
      • HSD 32 antibody
      • MGC102710 antibody
      • Rab escort protein 1 antibody
      • Rab geranylgeranyltransferase component A antibody
      • Rab proteins geranylgeranyltransferase component A 1 antibody
      • RAE1_HUMAN antibody
      • REP 1 antibody
      • REP-1 antibody
      • REP1 antibody
      • TCD antibody
      • TCD protein antibody
      see all

    Images

    • All lanes : Anti-CHM antibody [EPR7142] (ab134964) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Y79 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 73 kDa
      Observed band size: 95 kDa
      why is the actual band size different from the predicted?

    • Immunofluorescence analysis of CHM in A431 cells, using ab134964 at a dilution of 1/250.

    References

    ab134964 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    REP-1 siRNA
    Specification
    HEK293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Nov 08 2016

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Ovary)
    Antigen retrieval step
    Enzymatic
    Permeabilization
    No
    Specification
    Ovary
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 14 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up