Overview

  • Product name

  • Description

    Rabbit polyclonal to CHMP1a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Orangutan
  • Immunogen

    Recombinant full length protein corresponding to Human CHMP1a aa 1-196.
    Sequence:

    MDDTLFQLKFTAKQLEKLAKKAEKDSKAEQAKVKKALLQKNVECARVYAE NAIRKKNEGVNWLRMASRVDAVASKVQTAVTMKGVTKNMAQVTKALDKAL STMDLQKVSSVMDRFEQQVQNLDVHTSVMEDSMSSATTLTTPQEQVDSLI MQIAEENGLEVLDQLSQLPEGASAVGESSVRSQEDQLSRRLAALRN


    Database link: Q9HD42

  • Positive control

    • WB: HEK-293T whole cell lysate. IHC-P: Human testis and placenta tissues. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab237676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
ICC/IF 1/50 - 1/200.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing.
  • Tissue specificity

    Expressed in placenta, cultured skin fibroblasts and in osteoblast cell line MG-63.
  • Sequence similarities

    Belongs to the SNF7 family.
  • Cellular localization

    Cytoplasm. Endosome membrane. Nucleus matrix. The cytoplasmic form is partially membrane-associated and localizes to early endosomes. The nuclear form remains associated with the chromosome scaffold during mitosis. On overexpression, it localizes to nuclear bodies characterized by nuclease-resistant condensed chromatin.
  • Information by UniProt
  • Database links

  • Alternative names

    • Charged multivesicular body protein 1a antibody
    • CHM1A_HUMAN antibody
    • CHMP1a antibody
    • Chromatin modifying protein 1a antibody
    • Chromatin-modifying protein 1a antibody
    • hVps46 1 antibody
    • hVps46-1 antibody
    • KIAA0047 antibody
    • Metalloprotease 1 antibody
    • PCOLN3 antibody
    • Procollagen (type III) N endopeptidase antibody
    • PRSM1 antibody
    • Vacuolar protein sorting 46 1 antibody
    • Vacuolar protein sorting-associated protein 46-1 antibody
    • Vps46 1 antibody
    • Vps46-1 antibody
    see all

Images

  • Anti-CHMP1a antibody (ab237676) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 22 kDa

  • Paraffin-embedded human testis tissue stained for CHMP1a using ab237676 at 1/200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human placenta tissue stained for CHMP1a using ab237676 at 1/200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CHMP1a (green) using ab237676 at 1/66 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. 

References

ab237676 has not yet been referenced specifically in any publications.

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