Overview

  • Product name

    Anti-CHMP1a antibody [EPR11939]
    See all CHMP1a primary antibodies
  • Description

    Rabbit monoclonal [EPR11939] to CHMP1a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CHMP1a aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9HD42

  • Positive control

    • HeLa, 293T and MCF-7 cell lysates. Permeabilized 293T cells. Immunoprecipitation pellet from MCF-7 cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing.
    • Tissue specificity

      Expressed in placenta, cultured skin fibroblasts and in osteoblast cell line MG-63.
    • Sequence similarities

      Belongs to the SNF7 family.
    • Cellular localization

      Cytoplasm. Endosome membrane. Nucleus matrix. The cytoplasmic form is partially membrane-associated and localizes to early endosomes. The nuclear form remains associated with the chromosome scaffold during mitosis. On overexpression, it localizes to nuclear bodies characterized by nuclease-resistant condensed chromatin.
    • Information by UniProt
    • Database links

    • Alternative names

      • Charged multivesicular body protein 1a antibody
      • CHM1A_HUMAN antibody
      • CHMP1a antibody
      • Chromatin modifying protein 1a antibody
      • Chromatin-modifying protein 1a antibody
      • hVps46 1 antibody
      • hVps46-1 antibody
      • KIAA0047 antibody
      • Metalloprotease 1 antibody
      • PCOLN3 antibody
      • Procollagen (type III) N endopeptidase antibody
      • PRSM1 antibody
      • Vacuolar protein sorting 46 1 antibody
      • Vacuolar protein sorting-associated protein 46-1 antibody
      • Vps46 1 antibody
      • Vps46-1 antibody
      see all

    Images

    • All lanes : Anti-CHMP1a antibody [EPR11939] (ab178686) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : MCF-7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 22 kDa

    • Flow cytometric analysis of permeabilized 293T cells using ab178686 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Western blot analysis on immunoprecipitation pellet from (Lane 1) MCF-7 cell lysate or (Lane 2) 1XPBS (negative control) using ab178686 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab178686 has not yet been referenced specifically in any publications.

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