Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11939] to CHMP1a
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-CHMP1a antibody [EPR11939]
See all CHMP1a primary antibodies
DescriptionRabbit monoclonal [EPR11939] to CHMP1a
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CHMP1a aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9HD42
- HeLa, 293T and MCF-7 cell lysates. Permeabilized 293T cells. Immunoprecipitation pellet from MCF-7 cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab178686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 22 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
FunctionProbable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing.
Tissue specificityExpressed in placenta, cultured skin fibroblasts and in osteoblast cell line MG-63.
Sequence similaritiesBelongs to the SNF7 family.
Cellular localizationCytoplasm. Endosome membrane. Nucleus matrix. The cytoplasmic form is partially membrane-associated and localizes to early endosomes. The nuclear form remains associated with the chromosome scaffold during mitosis. On overexpression, it localizes to nuclear bodies characterized by nuclease-resistant condensed chromatin.
- Information by UniProt
- Charged multivesicular body protein 1a antibody
- CHM1A_HUMAN antibody
- CHMP1a antibody
All lanes : Anti-CHMP1a antibody [EPR11939] (ab178686) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : 293T cell lysates
Lane 3 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa
Flow cytometric analysis of permeabilized 293T cells using ab178686 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Western blot analysis on immunoprecipitation pellet from (Lane 1) MCF-7 cell lysate or (Lane 2) 1XPBS (negative control) using ab178686 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab178686 has not yet been referenced specifically in any publications.