Overview

  • Product name

  • Description

    Mouse polyclonal to CHMP2A/BC2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human CHMP2A/BC2 aa 1-222.

  • Positive control

    • Transfected 293T cell lysate
  • General notes

     This product was previously labelled as CHMP2A

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Predicted molecular weight: 25 kDa.
    Detection pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.
    • Sequence similarities

      Belongs to the SNF7 family.
    • Domain

      The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
    • Cellular localization

      Late endosome membrane. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
    • Information by UniProt
    • Database links

    • Alternative names

      • BC 2 antibody
      • BC2 antibody
      • Charged multivesicular body protein 2a antibody
      • CHM2A_HUMAN antibody
      • CHMP2 antibody
      • CHMP2a antibody
      • Chromatin modifying protein 2a antibody
      • Chromatin-modifying protein 2a antibody
      • hVps2 1 antibody
      • hVps2-1 antibody
      • putative breast adenocarcinoma marker antibody
      • Putative breast adenocarcinoma marker BC-2 antibody
      • Putative breast adenocarcinoma marker BC2 antibody
      • Vacuolar protein sorting associated protein 2 1 antibody
      • Vacuolar protein sorting-associated protein 2-1 antibody
      • Vps2 1 antibody
      • VPS2 antibody
      • Vps2-1 antibody
      • VPS2A antibody
      see all

    Images

    • All lanes : Anti-CHMP2A/BC2 antibody (ab67064) at 1/500 dilution

      Lane 1 : CHMP2A/BC2 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size: 25 kDa
      Observed band size: ~28 kDa
      why is the actual band size different from the predicted?

    References

    ab67064 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab67064.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up