Overview

  • Product name

  • Description

    Rabbit polyclonal to CHMP2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human CHMP2B aa 163-213. The exact sequence is proprietary. (NP_054762.2).
    Database link: Q9UQN3

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IHC-P: Human breast carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226304 was affinity purified using an epitope specific to CHMP2B immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 24 kDa.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.
    • Tissue specificity

      Widely expressed. Expressed in brain, heart, skeletal muscle, spleen, kidney, liver, small intestine, pancreas, lung, placenta and leukocytes. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal chord, occipital lobe, frontal lobe, temporal lobe and putamen.
    • Involvement in disease

      Defects in CHMP2B are the cause of frontotemporal dementia, chromosome 3-linked (FTD3) [MIM:600795]. FTD3 is characterized by an onset of dementia in the late 50's initially characterized by behavioral and personality changes including apathy, restlessness, disinhibition and hyperorality, progressing to stereotyped behaviors, non-fluent aphasia, mutism and dystonia, with a marked lack of insight. The brains of individuals with FTD3 have no distinctive neuropathological features. They show global cortical and central atrophy, but no beta-amyloid deposits.
    • Sequence similarities

      Belongs to the SNF7 family.
    • Domain

      The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
    • Cellular localization

      Cytoplasm > cytosol. Late endosome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ALS17 antibody
      • Charged multivesicular body protein 2b antibody
      • CHM2B_HUMAN antibody
      • CHMP family, member 2B antibody
      • CHMP2.5 antibody
      • CHMP2b antibody
      • Chromatin modifying protein 2b antibody
      • Chromatin-modifying protein 2b antibody
      • DMT1 antibody
      • hVps2-2 antibody
      • Vacuolar protein sorting 2, yeast, homolog of, B antibody
      • Vacuolar protein sorting 2-2 antibody
      • Vacuolar protein sorting-associated protein 2-2 antibody
      • VPS2 homolog B antibody
      • Vps2-2 antibody
      • VPS2B antibody
      see all

    Images

    • All lanes : Anti-CHMP2B antibody (ab226304) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
      Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg
      Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 24 kDa


      Exposure time: 30 seconds


      Cell lysates were prepared using NETN lysis buffer.

    • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CHMP2B using ab226304 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: Hematoxylin (blue).

    References

    ab226304 has not yet been referenced specifically in any publications.

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