Key features and details
- Rabbit polyclonal to CHMP2B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CHMP2B antibody
See all CHMP2B primary antibodies
DescriptionRabbit polyclonal to CHMP2B
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Orangutan, Xenopus tropicalis
Synthetic peptide within Human CHMP2B aa 163-213. The exact sequence is proprietary. (NP_054762.2).
Database link: Q9UQN3
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IHC-P: Human breast carcinoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226304 was affinity purified using an epitope specific to CHMP2B immobilized on solid support.
Our Abpromise guarantee covers the use of ab226304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 24 kDa.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProbable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.
Tissue specificityWidely expressed. Expressed in brain, heart, skeletal muscle, spleen, kidney, liver, small intestine, pancreas, lung, placenta and leukocytes. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal chord, occipital lobe, frontal lobe, temporal lobe and putamen.
Involvement in diseaseDefects in CHMP2B are the cause of frontotemporal dementia, chromosome 3-linked (FTD3) [MIM:600795]. FTD3 is characterized by an onset of dementia in the late 50's initially characterized by behavioral and personality changes including apathy, restlessness, disinhibition and hyperorality, progressing to stereotyped behaviors, non-fluent aphasia, mutism and dystonia, with a marked lack of insight. The brains of individuals with FTD3 have no distinctive neuropathological features. They show global cortical and central atrophy, but no beta-amyloid deposits.
Sequence similaritiesBelongs to the SNF7 family.
DomainThe acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
Cellular localizationCytoplasm > cytosol. Late endosome membrane.
- Information by UniProt
- ALS17 antibody
- Charged multivesicular body protein 2b antibody
- CHM2B_HUMAN antibody
All lanes : Anti-CHMP2B antibody (ab226304) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 30 seconds
Cell lysates were prepared using NETN lysis buffer.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CHMP2B using ab226304 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: Hematoxylin (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226304 has not yet been referenced specifically in any publications.