Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (mouse brain, cortex)
Specification
mouse brain, cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS containing 0,1% Tween20 and 5% nonfa
Dilution
1/1500
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit IgG (Dako)
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Bands
Specific: approx. 29 kDa Non-specific: approx. 51 kDa
Exposure
2 minute(s) and 0 second(s)
Additional data
Additional Notes
nitrocellulose membrane
Protease inhibitors: 1 tablet Protease Inhibitor Cocktail (Roche)
Attached image:
lanes 1,2,3,4 C57Bl6 mouse brain
lanes 1,2: ab33174, 1/1500
lanes 3,4: ab33174, 1/3000
lane 5: marker Invitrogen Benchmark (10 KDa steps and 25 kDa, 50kDa indicated)
Abcam user community
Verified customer
Submitted May 15 2014