Overview

  • Product name

    Anti-CHMP2B antibody [EPR10807(B)]
    See all CHMP2B primary antibodies
  • Description

    Rabbit monoclonal [EPR10807(B)] to CHMP2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human CHMP2B (Uniprot: Q9UQN3)

  • Positive control

    • Recombinant Human CHMP2B protein (ab109959) can be used as a positive control in WB. HepG2, A431 and 293T cell lysates; fetal brain lysate; HepG2 and 293T cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.
    • Tissue specificity

      Widely expressed. Expressed in brain, heart, skeletal muscle, spleen, kidney, liver, small intestine, pancreas, lung, placenta and leukocytes. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal chord, occipital lobe, frontal lobe, temporal lobe and putamen.
    • Involvement in disease

      Defects in CHMP2B are the cause of frontotemporal dementia, chromosome 3-linked (FTD3) [MIM:600795]. FTD3 is characterized by an onset of dementia in the late 50's initially characterized by behavioral and personality changes including apathy, restlessness, disinhibition and hyperorality, progressing to stereotyped behaviors, non-fluent aphasia, mutism and dystonia, with a marked lack of insight. The brains of individuals with FTD3 have no distinctive neuropathological features. They show global cortical and central atrophy, but no beta-amyloid deposits.
    • Sequence similarities

      Belongs to the SNF7 family.
    • Domain

      The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
    • Cellular localization

      Cytoplasm > cytosol. Late endosome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ALS17 antibody
      • Charged multivesicular body protein 2b antibody
      • CHM2B_HUMAN antibody
      • CHMP family, member 2B antibody
      • CHMP2.5 antibody
      • CHMP2b antibody
      • Chromatin modifying protein 2b antibody
      • Chromatin-modifying protein 2b antibody
      • DMT1 antibody
      • hVps2-2 antibody
      • Vacuolar protein sorting 2, yeast, homolog of, B antibody
      • Vacuolar protein sorting 2-2 antibody
      • Vacuolar protein sorting-associated protein 2-2 antibody
      • VPS2 homolog B antibody
      • Vps2-2 antibody
      • VPS2B antibody
      see all

    Images

    • All lanes : Anti-CHMP2B antibody [EPR10807(B)] (ab157208) at 1/1000 dilution

      Lane 1 : Wild-type A549 whole cell lysate
      Lane 2 : CHMP2B knockout A549 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : MCF7 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 24 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab157208 observed at 45 kDa. Red - loading control, ab130007, observed at 130 kDa.

      ab157208 was shown to specifically react with CHMP2B in wild-type A549 cells as signal was lost in CHMP2B knockout cells. Wild-type and CHMP2B knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab157208 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunofluorescence analysis of HepG2 cells, labeling CHMP2B using ab157208 at a 1/250 dilution.
    • All lanes : Anti-CHMP2B antibody [EPR10807(B)] (ab157208) at 1/1000 dilution

      Lane 1 : fetal brain lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 24 kDa

    • Western blot analysis of Immunoprecipitation pellet from Human fetal brain lysate showing CHMP2B, using ab157208 at 1/10 dilution for IP
    • Flow cytometry analysis of permeabilized 293T cells labeling CHMP2B (red), using ab157208 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)

    References

    ab157208 has not yet been referenced specifically in any publications.

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