Key features and details
- Mouse polyclonal to CHMP4A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CHMP4A antibody
DescriptionMouse polyclonal to CHMP4A
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length protein, corresponding to amino acids 1-223 of Human CHMP4A
- transfected 293T cells lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab67058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 25 kDa. Use BSA for blocking (see Abreview)|
RelevanceComponent of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In the ESCRT-III complex, it probably serves as an acceptor for ESCRT-I complex on endosomal membranes. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins, via the association of CHMP4 proteins with PDCD6IP/AIP1, a protein directly recruited by HIV-1 p6 protein that functions at sites of viral Gag assembly and budding.
Cellular localizationCytoplasmic: Cytoplasmic vesicle membrane. Membrane-associated - localizes to large vesicle-like structures.
- C14orf123 antibody
- Charged multivesicular body protein 4a antibody
- Chromatin modifying protein 4a antibody
All lanes : Anti-CHMP4A antibody (ab67058) at 1/500 dilution
Lane 1 : CHMP4A transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 25 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
ab67058 has been referenced in 1 publication.
- Arii J et al. ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity. Nat Commun 9:3379 (2018). PubMed: 30139939