Overview

  • Product name

    Anti-CHMP4A antibody
  • Description

    Mouse polyclonal to CHMP4A
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-223 of Human CHMP4A

  • Positive control

    • transfected 293T cells lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa. Use BSA for blocking (see Abreview)

Target

  • Relevance

    Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In the ESCRT-III complex, it probably serves as an acceptor for ESCRT-I complex on endosomal membranes. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins, via the association of CHMP4 proteins with PDCD6IP/AIP1, a protein directly recruited by HIV-1 p6 protein that functions at sites of viral Gag assembly and budding.
  • Cellular localization

    Cytoplasmic: Cytoplasmic vesicle membrane. Membrane-associated - localizes to large vesicle-like structures.
  • Database links

  • Alternative names

    • C14orf123 antibody
    • Charged multivesicular body protein 4a antibody
    • Chromatin modifying protein 4a antibody
    • HSPC134 antibody
    • SHAX2 antibody
    see all

Images

  • All lanes : Anti-CHMP4A antibody (ab67058) at 1/500 dilution

    Lane 1 : CHMP4A transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size: 25 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Arii J  et al. ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity. Nat Commun 9:3379 (2018). Read more (PubMed: 30139939) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
30000 cells
Specification
293T
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted May 27 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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