Overview

  • Product name

  • Description

    Rabbit polyclonal to CHMP4B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Human CHMP4B protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab135154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/5000. Predicted molecular weight: 25 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/100 - 1/2000.

Target

  • Function

    Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6-and p9-dependent virus release.
  • Tissue specificity

    Widely expressed. Expressed at higher level in heart and skeletal muscle. Also expressed in brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.
  • Involvement in disease

    Defects in CHMP4B are the cause of cataract posterior polar type 3 (CTPP3) [MIM:605387]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity.
  • Sequence similarities

    Belongs to the SNF7 family.
  • Domain

    The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • Cellular localization

    Cytoplasm > cytosol. Late endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Charged multivesicular body protein 4b antibody
    • CHM4B_HUMAN antibody
    • chmp4b antibody
    • Chromatin-modifying protein 4b antibody
    • hSnf7-2 antibody
    • hVps32-2 antibody
    • SNF7 homolog associated with Alix 1 antibody
    • SNF7-2 antibody
    • Vacuolar protein sorting-associated protein 32-2 antibody
    • Vps32-2 antibody
    see all

References

ab135154 has not yet been referenced specifically in any publications.

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