Overview

  • Product name
  • Description
    Rabbit polyclonal to CHMP4C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-168 of Human CHMP4C (Uniprot ID: Q96CF2).

  • Positive control
    • HeLa whole cell and cytoplasmic lysates, Mouse kidney lysate; Human U87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.
  • Tissue specificity
    Expressed in heart, spleen and kidney.
  • Sequence similarities
    Belongs to the SNF7 family.
  • Domain
    The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • Cellular localization
    Cytoplasm > cytosol. Late endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Charged multivesicular body protein 4c antibody
    • CHM4C_HUMAN antibody
    • CHMP4c antibody
    • Chromatin-modifying protein 4c antibody
    • hSnf7-3 antibody
    • hVps32-3 antibody
    • SHAX3 antibody
    • SNF7 homolog associated with Alix 3 antibody
    • SNF7-3 antibody
    • Vacuolar protein sorting-associated protein 32-3 antibody
    • Vps32-3 antibody
    see all

Images

  • Anti-CHMP4C antibody (ab155668) at 1/1000 dilution + Mouse kidney whole cell lysate at 50 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • All lanes : Anti-CHMP4C antibody (ab155668) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa cytoplasmic lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue labeling CHMP4C with ab155668 at 1/500 dilution.

References

This product has been referenced in:
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Loading amount
80 µg
Gel Running Conditions
Non-reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (Colon carcinoma)
Specification
Colon carcinoma
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Dr. Eleni Petsalaki

Verified customer

Submitted Oct 29 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Human Cell (Colon cancer)
Specification
Colon cancer
Permeabilization
No
Fixative
Methanol

Dr. Eleni Petsalaki

Verified customer

Submitted Oct 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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