Key features and details
- Rabbit polyclonal to CHMP5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CHMP5 antibody
DescriptionRabbit polyclonal to CHMP5
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Zebrafish
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 172 of Human CHMP5
- 293T, A431, H1299, HeLa S3, HepG2 whole cell lysate (ab7900) (WB). NCIN87 xenograft (IHC).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 25 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionProbable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.
Sequence similaritiesBelongs to the SNF7 family.
Cellular localizationCytoplasm > cytosol. Endosome membrane. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
- Information by UniProt
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All lanes : Anti-CHMP5 antibody (ab96273) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa
12% SDS PAGE
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ab96273 at 1/100 dilution
ab96273 has been referenced in 1 publication.
- Son F et al. Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting. Methods Mol Biol 1998:219-226 (2019). PubMed: 31250305